High-Resolution 3-D Imaging of Biological Objects by Standing-Wave Fluorescence Microscopy

Brent Bailey; Frederick Lanni

doi:10.1364/OAM.1993.MG.4

OSA Annual Meeting
Technical Digest Series (Optica Publishing Group, 1993),
paper MG.4
•https://doi.org/10.1364/OAM.1993.MG.4

High-Resolution 3-D Imaging of Biological Objects by Standing-Wave Fluorescence Microscopy

Brent Bailey and Frederick Lanni

OSA Annual Meeting 1993

Toronto Canada
3–8 October 1993
ISBN:

From the session
Symposium on optical imaging of biological and medical media (MG)

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B. Bailey and F. Lanni, "High-Resolution 3-D Imaging of Biological Objects by Standing-Wave Fluorescence Microscopy," in OSA Annual Meeting, Technical Digest Series (Optica Publishing Group, 1993), paper MG.4.

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Abstract

Axial resolution in conventional fluorescence microscopy is considerably worse than transverse resolution because the axial location of a specimen feature is not encoded directly in the image, but rather, is indicated by its degree of defocus.

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