High resolution 3D imaging of primary and secondary tumor spheroids using multicolor multi-angle Light Sheet Fluorescence Microscopy (LSFM)

Stylianos E. Psycharakis; Stylianos E. Psycharakis; Stylianos E. Psycharakis; Evangelos Liapis; Evangelos Liapis; Evangelos Liapis; Athanasios Zacharopoulos; Athanasios Zacharopoulos; Mariam-Eleni Oraiopoulou; Mariam-Eleni Oraiopoulou; Chrysoula Aivalioti; Chrysoula Aivalioti; Vangelis Sakkalis; Vangelis Sakkalis; Joseph Papamatheakis; Joseph Papamatheakis; Jorge Ripoll; Jorge Ripoll; Giannis Zacharakis; Giannis Zacharakis

doi:10.1117/12.2527214

Advances in Microscopic Imaging II
SPIE Proceedings (Optica Publishing Group, 2019),
paper 11076_24
•https://doi.org/10.1117/12.2527214

High resolution 3D imaging of primary and secondary tumor spheroids using multicolor multi-angle Light Sheet Fluorescence Microscopy (LSFM)

Stylianos E. Psycharakis, Evangelos Liapis, Athanasios Zacharopoulos, Mariam-Eleni Oraiopoulou, Chrysoula Aivalioti, Vangelis Sakkalis, Joseph Papamatheakis, Jorge Ripoll, and Giannis Zacharakis

European Conference on Biomedical Optics 2019

Munich Germany
23–25 June 2019
ISBN: 9781510628458

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Excitation Shaping and Advanced Methods (ESAM)

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S. E. Psycharakis, E. Liapis, A. Zacharopoulos, M. Oraiopoulou, C. Aivalioti, V. Sakkalis, J. Papamatheakis, J. Ripoll, and G. Zacharakis, "High resolution 3D imaging of primary and secondary tumor spheroids using multicolor multi-angle Light Sheet Fluorescence Microscopy (LSFM)," in Advances in Microscopic Imaging II, Vol. EB103 of SPIE Proceedings (Optica Publishing Group, 2019), paper 11076_24.

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Abstract

Breast cancer and Glioblastoma brain cancer are aggressive malignancies with poor prognosis. In this study primary Glioblastoma and secondary breast cancer spheroids are formed and treated with the well-known Temozolomide and Doxorubicin chemotherapeutics, respectively. A custom multi-angle Light Sheet Fluorescence Microscope is employed for high resolution imaging of both cancer cell spheroids. Such a technique is successful in realizing pre-clinical drug screening, while enables the discrimination among physiologic tumor parameters. LSFM technique, parameters and method followed are also presented.

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