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Conferences > ECBO > 2003 > MIII > 5143_139

Fluorescence apertureless scanning near-field microscopy for high resolution biological imaging

A. Fragola and L. Aigouy

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Abstract

Apertureless scanning near-field optical microscopy (SNOM) offers new opportunities in fluorescence imaging by providing subwavelength resolution. This is achieved by scattering the near-field with a metallic tip. SNOM images recorded on sub-micron sized fluorescent spheres show a subwavelength resolution and an interesting interference phenomenon that we modelized in detail. We will show that the microscope is suitable for immersed samples imaging, besides an essential need for the study of biological samples.

© 2003 SPIE

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