Abstract
Optical microscopy has been widely used as a versatile tool in biological research. However, its penetration depth and spatial resolution are desperately limited by light scattering during deep propagation in turbid medium. Here, we implement near-infrared second window (1000–1700 nm) multifocal structured illumination microscopy (NIR-II MSIM) capable of deep penetration, high contrast, and enhanced spatial resolution. Raster-scanning multifocal illumination patterns ensure homogeneous illumination of the sample. By integrating NIR-II photoemission into multifocal photoexcitation, NIR-II MSIM affords deep imaging with improved lateral resolution (∼1.49 µm) at a depth of 2.5 mm in an Intralipid/agar phantom and outstanding contrast. Additionally, imaging at longer wavelength in the NIR-II region shows superior performance. This NIR-II MSIM system will afford a promising platform for studying physiological phenomena in turbid specimens in the future.
© 2022 Optica Publishing Group
Full Article | PDF ArticleMore Like This
Yong Zhang, Chenshuang Zhang, Renlong Zhang, Rong Xu, Bin Yu, Danying Lin, and Junle Qu
Opt. Lett. 49(6) 1540-1543 (2024)
Jingjing Wu, Siwei Li, Huiqun Cao, Danying Lin, Bin Yu, and Junle Qu
Opt. Express 26(24) 31430-31438 (2018)
Wei Zhang, Bin Yu, Danying Lin, Huanhuan Yu, Siwei Li, and Junle Qu
Opt. Express 28(8) 10919-10927 (2020)