Add to BibSonomy
Abstract
In this Letter, we present a single-shot 3D-resolved structured illumination microscopy (SIM) based on a digital micromirror device (DMD), a galvanometric mirror, and the HiLo algorithm. During imaging, the DMD rapidly generates sinusoidal and plane illuminations in the focal region. By synchronizing the DMD with a galvanometric scanner and exploiting the unique data readout process of the camera, the emissions from the specimen under two different illuminations, i.e., structured and uniform illumination, are projected to different regions on a camera, achieving high-resolution single-exposure optical sectioning at the camera’s limiting speed, i.e., 200 Hz, without sacrificing the resolution. A model has been developed to guide the design and optimization of the optical system. Imaging experiments on pollen and mouse kidney samples have been performed to verify the predicted performance. The results show that the single-shot SIM with the HiLo algorithm achieves comparable resolution to the standard two-shot HiLo method with a twofold speed enhancement, which may find important applications in biophotonics, e.g., visualizing high-speed biological events in vivo.
© 2022 Optica Publishing Group
Full Article | PDF ArticleMore Like This
Zhiqiang Fu, Qiang Geng, Jialong Chen, Li-An Chu, Ann-Shyn Chiang, and Shih-Chi Chen
Opt. Lett. 46(14) 3424-3427 (2021)
Xiaolong Cheng, Yan Tang, Kejun Yang, Lei Liu, and Chenhaolei Han
Opt. Lett. 47(15) 3832-3835 (2022)
Changchun Chai, Cheng Chen, Xiaojun Liu, and ZiLi Lei
Opt. Express 29(3) 4010-4021 (2021)