Abstract

Multifocal structured illumination microscopy (MSIM) is the parallelized version of image scanning microscopy (ISM), which uses multiple diffraction limited spots, instead of a single diffraction limited spot, to increase the imaging speed. By adding pinhole, contraction and deconvolution, a twofold resolution enhancement could be achieved in theory. However, this resolution improvement is difficult to be attained in practice. In this work, without any modification of the experimental setup, we propose to use multiple measurement vector (MMV) model sparse Bayesian learning (MSBL) algorithm (MSIMMSBL) as the reconstruction algorithm of MSIM, which does not need to estimate the illumination patterns but treat the reconstruct process as an MMV signal reconstruction problem. We compare the reconstructed super-resolution images of MSIMMSBL and MSIM by using simulation and experimental raw images. The results prove that by using the MSBL algorithm, the MSIM can obtain a higher than twofold resolution enhancement compared with the wide field image. This outstanding imaging resolution combining with the primary advantages of MSIM, such as the high imaging speed, could promote the application of MSIM in super-resolution microscopy imaging technology.

© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

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References

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  1. M. G. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc. 198(2), 82–87 (2000).
    [Crossref] [PubMed]
  2. A. G. York, P. Chandris, D. D. Nogare, J. Head, P. Wawrzusin, R. S. Fischer, A. Chitnis, and H. Shroff, “Instant super-resolution imaging in live cells and embryos via analog image processing,” Nat. Methods 10(11), 1122–1126 (2013).
    [Crossref] [PubMed]
  3. A. G. York, S. H. Parekh, D. Dalle Nogare, R. S. Fischer, K. Temprine, M. Mione, A. B. Chitnis, C. A. Combs, and H. Shroff, “Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy,” Nat. Methods 9(7), 749–754 (2012).
    [Crossref] [PubMed]
  4. P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
    [Crossref] [PubMed]
  5. L. Shao, P. Kner, E. H. Rego, and M. G. Gustafsson, “Super-resolution 3D microscopy of live whole cells using structured illumination,” Nat. Methods 8(12), 1044–1046 (2011).
    [Crossref] [PubMed]
  6. C. B. Müller and J. Enderlein, “Image scanning microscopy,” Phys. Rev. Lett. 104(19), 198101 (2010).
    [Crossref] [PubMed]
  7. E. N. Ward, F. H. Torkelsen, and R. Pal, “Enhancing multi-spot structured illumination microscopy with fluorescence difference,” R. Soc. Open Sci. 5(3), 171336 (2018).
    [Crossref] [PubMed]
  8. M. Ingaramo, A. G. York, P. Wawrzusin, O. Milberg, A. Hong, R. Weigert, H. Shroff, and G. H. Patterson, “Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue,” Proc. Natl. Acad. Sci. U.S.A. 111(14), 5254–5259 (2014).
    [Crossref] [PubMed]
  9. F. Ströhl and C. F. Kaminski, “A joint Richardson-Lucy deconvolution algorithm for the reconstruction of multifocal structured illumination microscopy data,” Methods Appl. Fluoresc. 3(1), 014002 (2015).
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    [Crossref] [PubMed]
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    [Crossref]
  13. N. Han and Z. Song, “Bayesian multiple measurement vector problem with spatial structured sparsity patterns,” Digit. Signal Process. 75, 184–201 (2018).
    [Crossref]
  14. J. Min, J. Jang, D. Keum, S. W. Ryu, C. Choi, K. H. Jeong, and J. C. Ye, “Fluorescent microscopy beyond diffraction limits using speckle illumination and joint support recovery,” Sci. Rep. 3(1), 2075 (2013).
    [Crossref] [PubMed]
  15. J. Wu, S. Li, S. Zhang, D. Lin, B. Yu, and J. Qu, “Fast analysis method for stochastic optical reconstruction microscopy using multiple measurement vector model sparse Bayesian learning,” Opt. Lett. 43(16), 3977–3980 (2018).
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    [Crossref] [PubMed]

2018 (4)

E. N. Ward, F. H. Torkelsen, and R. Pal, “Enhancing multi-spot structured illumination microscopy with fluorescence difference,” R. Soc. Open Sci. 5(3), 171336 (2018).
[Crossref] [PubMed]

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

N. Han and Z. Song, “Bayesian multiple measurement vector problem with spatial structured sparsity patterns,” Digit. Signal Process. 75, 184–201 (2018).
[Crossref]

J. Wu, S. Li, S. Zhang, D. Lin, B. Yu, and J. Qu, “Fast analysis method for stochastic optical reconstruction microscopy using multiple measurement vector model sparse Bayesian learning,” Opt. Lett. 43(16), 3977–3980 (2018).
[Crossref] [PubMed]

2017 (1)

2015 (1)

F. Ströhl and C. F. Kaminski, “A joint Richardson-Lucy deconvolution algorithm for the reconstruction of multifocal structured illumination microscopy data,” Methods Appl. Fluoresc. 3(1), 014002 (2015).
[Crossref] [PubMed]

2014 (1)

M. Ingaramo, A. G. York, P. Wawrzusin, O. Milberg, A. Hong, R. Weigert, H. Shroff, and G. H. Patterson, “Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue,” Proc. Natl. Acad. Sci. U.S.A. 111(14), 5254–5259 (2014).
[Crossref] [PubMed]

2013 (2)

A. G. York, P. Chandris, D. D. Nogare, J. Head, P. Wawrzusin, R. S. Fischer, A. Chitnis, and H. Shroff, “Instant super-resolution imaging in live cells and embryos via analog image processing,” Nat. Methods 10(11), 1122–1126 (2013).
[Crossref] [PubMed]

J. Min, J. Jang, D. Keum, S. W. Ryu, C. Choi, K. H. Jeong, and J. C. Ye, “Fluorescent microscopy beyond diffraction limits using speckle illumination and joint support recovery,” Sci. Rep. 3(1), 2075 (2013).
[Crossref] [PubMed]

2012 (2)

E. Mudry, K. Belkebir, J. Girard, J. Savatier, E. Le Moal, C. Nicoletti, M. Allain, and A. Sentenac, “Structured illumination microscopy using unknown speckle patterns,” Nat. Photonics 6(5), 312–315 (2012).
[Crossref]

A. G. York, S. H. Parekh, D. Dalle Nogare, R. S. Fischer, K. Temprine, M. Mione, A. B. Chitnis, C. A. Combs, and H. Shroff, “Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy,” Nat. Methods 9(7), 749–754 (2012).
[Crossref] [PubMed]

2011 (1)

L. Shao, P. Kner, E. H. Rego, and M. G. Gustafsson, “Super-resolution 3D microscopy of live whole cells using structured illumination,” Nat. Methods 8(12), 1044–1046 (2011).
[Crossref] [PubMed]

2010 (1)

C. B. Müller and J. Enderlein, “Image scanning microscopy,” Phys. Rev. Lett. 104(19), 198101 (2010).
[Crossref] [PubMed]

2009 (2)

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[Crossref] [PubMed]

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A. 106(52), 22287–22292 (2009).
[Crossref] [PubMed]

2007 (1)

D. P. Wipf and B. D. Rao, “An empirical Bayesian strategy for solving the simultaneous sparse approximation problem,” IEEE Trans. Signal Process. 55(7), 3704–3716 (2007).
[Crossref]

2000 (1)

M. G. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc. 198(2), 82–87 (2000).
[Crossref] [PubMed]

Allain, M.

E. Mudry, K. Belkebir, J. Girard, J. Savatier, E. Le Moal, C. Nicoletti, M. Allain, and A. Sentenac, “Structured illumination microscopy using unknown speckle patterns,” Nat. Photonics 6(5), 312–315 (2012).
[Crossref]

Belkebir, K.

E. Mudry, K. Belkebir, J. Girard, J. Savatier, E. Le Moal, C. Nicoletti, M. Allain, and A. Sentenac, “Structured illumination microscopy using unknown speckle patterns,” Nat. Photonics 6(5), 312–315 (2012).
[Crossref]

Cao, R.

Chandris, P.

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

A. G. York, P. Chandris, D. D. Nogare, J. Head, P. Wawrzusin, R. S. Fischer, A. Chitnis, and H. Shroff, “Instant super-resolution imaging in live cells and embryos via analog image processing,” Nat. Methods 10(11), 1122–1126 (2013).
[Crossref] [PubMed]

Chen, J.

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

Chhun, B. B.

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[Crossref] [PubMed]

Chitnis, A.

A. G. York, P. Chandris, D. D. Nogare, J. Head, P. Wawrzusin, R. S. Fischer, A. Chitnis, and H. Shroff, “Instant super-resolution imaging in live cells and embryos via analog image processing,” Nat. Methods 10(11), 1122–1126 (2013).
[Crossref] [PubMed]

Chitnis, A. B.

A. G. York, S. H. Parekh, D. Dalle Nogare, R. S. Fischer, K. Temprine, M. Mione, A. B. Chitnis, C. A. Combs, and H. Shroff, “Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy,” Nat. Methods 9(7), 749–754 (2012).
[Crossref] [PubMed]

Choi, C.

J. Min, J. Jang, D. Keum, S. W. Ryu, C. Choi, K. H. Jeong, and J. C. Ye, “Fluorescent microscopy beyond diffraction limits using speckle illumination and joint support recovery,” Sci. Rep. 3(1), 2075 (2013).
[Crossref] [PubMed]

Colyer, R.

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A. 106(52), 22287–22292 (2009).
[Crossref] [PubMed]

Combs, C. A.

A. G. York, S. H. Parekh, D. Dalle Nogare, R. S. Fischer, K. Temprine, M. Mione, A. B. Chitnis, C. A. Combs, and H. Shroff, “Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy,” Nat. Methods 9(7), 749–754 (2012).
[Crossref] [PubMed]

Dalle Nogare, D.

A. G. York, S. H. Parekh, D. Dalle Nogare, R. S. Fischer, K. Temprine, M. Mione, A. B. Chitnis, C. A. Combs, and H. Shroff, “Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy,” Nat. Methods 9(7), 749–754 (2012).
[Crossref] [PubMed]

Dertinger, T.

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A. 106(52), 22287–22292 (2009).
[Crossref] [PubMed]

Enderlein, J.

C. B. Müller and J. Enderlein, “Image scanning microscopy,” Phys. Rev. Lett. 104(19), 198101 (2010).
[Crossref] [PubMed]

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A. 106(52), 22287–22292 (2009).
[Crossref] [PubMed]

Fang, Y.

Fischer, R.

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

Fischer, R. S.

A. G. York, P. Chandris, D. D. Nogare, J. Head, P. Wawrzusin, R. S. Fischer, A. Chitnis, and H. Shroff, “Instant super-resolution imaging in live cells and embryos via analog image processing,” Nat. Methods 10(11), 1122–1126 (2013).
[Crossref] [PubMed]

A. G. York, S. H. Parekh, D. Dalle Nogare, R. S. Fischer, K. Temprine, M. Mione, A. B. Chitnis, C. A. Combs, and H. Shroff, “Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy,” Nat. Methods 9(7), 749–754 (2012).
[Crossref] [PubMed]

Giannini, J. P.

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

Girard, J.

E. Mudry, K. Belkebir, J. Girard, J. Savatier, E. Le Moal, C. Nicoletti, M. Allain, and A. Sentenac, “Structured illumination microscopy using unknown speckle patterns,” Nat. Photonics 6(5), 312–315 (2012).
[Crossref]

Griffis, E. R.

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[Crossref] [PubMed]

Guo, M.

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

Gustafsson, M. G.

L. Shao, P. Kner, E. H. Rego, and M. G. Gustafsson, “Super-resolution 3D microscopy of live whole cells using structured illumination,” Nat. Methods 8(12), 1044–1046 (2011).
[Crossref] [PubMed]

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[Crossref] [PubMed]

M. G. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc. 198(2), 82–87 (2000).
[Crossref] [PubMed]

Han, N.

N. Han and Z. Song, “Bayesian multiple measurement vector problem with spatial structured sparsity patterns,” Digit. Signal Process. 75, 184–201 (2018).
[Crossref]

Head, J.

A. G. York, P. Chandris, D. D. Nogare, J. Head, P. Wawrzusin, R. S. Fischer, A. Chitnis, and H. Shroff, “Instant super-resolution imaging in live cells and embryos via analog image processing,” Nat. Methods 10(11), 1122–1126 (2013).
[Crossref] [PubMed]

Hong, A.

M. Ingaramo, A. G. York, P. Wawrzusin, O. Milberg, A. Hong, R. Weigert, H. Shroff, and G. H. Patterson, “Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue,” Proc. Natl. Acad. Sci. U.S.A. 111(14), 5254–5259 (2014).
[Crossref] [PubMed]

Ingaramo, M.

M. Ingaramo, A. G. York, P. Wawrzusin, O. Milberg, A. Hong, R. Weigert, H. Shroff, and G. H. Patterson, “Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue,” Proc. Natl. Acad. Sci. U.S.A. 111(14), 5254–5259 (2014).
[Crossref] [PubMed]

Iyer, G.

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A. 106(52), 22287–22292 (2009).
[Crossref] [PubMed]

Jang, J.

J. Min, J. Jang, D. Keum, S. W. Ryu, C. Choi, K. H. Jeong, and J. C. Ye, “Fluorescent microscopy beyond diffraction limits using speckle illumination and joint support recovery,” Sci. Rep. 3(1), 2075 (2013).
[Crossref] [PubMed]

Jeong, K. H.

J. Min, J. Jang, D. Keum, S. W. Ryu, C. Choi, K. H. Jeong, and J. C. Ye, “Fluorescent microscopy beyond diffraction limits using speckle illumination and joint support recovery,” Sci. Rep. 3(1), 2075 (2013).
[Crossref] [PubMed]

Kaminski, C. F.

F. Ströhl and C. F. Kaminski, “A joint Richardson-Lucy deconvolution algorithm for the reconstruction of multifocal structured illumination microscopy data,” Methods Appl. Fluoresc. 3(1), 014002 (2015).
[Crossref] [PubMed]

Keum, D.

J. Min, J. Jang, D. Keum, S. W. Ryu, C. Choi, K. H. Jeong, and J. C. Ye, “Fluorescent microscopy beyond diffraction limits using speckle illumination and joint support recovery,” Sci. Rep. 3(1), 2075 (2013).
[Crossref] [PubMed]

Kner, P.

L. Shao, P. Kner, E. H. Rego, and M. G. Gustafsson, “Super-resolution 3D microscopy of live whole cells using structured illumination,” Nat. Methods 8(12), 1044–1046 (2011).
[Crossref] [PubMed]

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[Crossref] [PubMed]

Kuang, C.

Le Moal, E.

E. Mudry, K. Belkebir, J. Girard, J. Savatier, E. Le Moal, C. Nicoletti, M. Allain, and A. Sentenac, “Structured illumination microscopy using unknown speckle patterns,” Nat. Photonics 6(5), 312–315 (2012).
[Crossref]

Li, S.

Lin, D.

Liu, X.

Milberg, O.

M. Ingaramo, A. G. York, P. Wawrzusin, O. Milberg, A. Hong, R. Weigert, H. Shroff, and G. H. Patterson, “Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue,” Proc. Natl. Acad. Sci. U.S.A. 111(14), 5254–5259 (2014).
[Crossref] [PubMed]

Min, J.

J. Min, J. Jang, D. Keum, S. W. Ryu, C. Choi, K. H. Jeong, and J. C. Ye, “Fluorescent microscopy beyond diffraction limits using speckle illumination and joint support recovery,” Sci. Rep. 3(1), 2075 (2013).
[Crossref] [PubMed]

Mione, M.

A. G. York, S. H. Parekh, D. Dalle Nogare, R. S. Fischer, K. Temprine, M. Mione, A. B. Chitnis, C. A. Combs, and H. Shroff, “Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy,” Nat. Methods 9(7), 749–754 (2012).
[Crossref] [PubMed]

Mudry, E.

E. Mudry, K. Belkebir, J. Girard, J. Savatier, E. Le Moal, C. Nicoletti, M. Allain, and A. Sentenac, “Structured illumination microscopy using unknown speckle patterns,” Nat. Photonics 6(5), 312–315 (2012).
[Crossref]

Müller, C. B.

C. B. Müller and J. Enderlein, “Image scanning microscopy,” Phys. Rev. Lett. 104(19), 198101 (2010).
[Crossref] [PubMed]

Nicoletti, C.

E. Mudry, K. Belkebir, J. Girard, J. Savatier, E. Le Moal, C. Nicoletti, M. Allain, and A. Sentenac, “Structured illumination microscopy using unknown speckle patterns,” Nat. Photonics 6(5), 312–315 (2012).
[Crossref]

Nogare, D. D.

A. G. York, P. Chandris, D. D. Nogare, J. Head, P. Wawrzusin, R. S. Fischer, A. Chitnis, and H. Shroff, “Instant super-resolution imaging in live cells and embryos via analog image processing,” Nat. Methods 10(11), 1122–1126 (2013).
[Crossref] [PubMed]

Pal, R.

E. N. Ward, F. H. Torkelsen, and R. Pal, “Enhancing multi-spot structured illumination microscopy with fluorescence difference,” R. Soc. Open Sci. 5(3), 171336 (2018).
[Crossref] [PubMed]

Parekh, S. H.

A. G. York, S. H. Parekh, D. Dalle Nogare, R. S. Fischer, K. Temprine, M. Mione, A. B. Chitnis, C. A. Combs, and H. Shroff, “Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy,” Nat. Methods 9(7), 749–754 (2012).
[Crossref] [PubMed]

Patterson, G. H.

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

M. Ingaramo, A. G. York, P. Wawrzusin, O. Milberg, A. Hong, R. Weigert, H. Shroff, and G. H. Patterson, “Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue,” Proc. Natl. Acad. Sci. U.S.A. 111(14), 5254–5259 (2014).
[Crossref] [PubMed]

Qu, J.

Rao, B. D.

D. P. Wipf and B. D. Rao, “An empirical Bayesian strategy for solving the simultaneous sparse approximation problem,” IEEE Trans. Signal Process. 55(7), 3704–3716 (2007).
[Crossref]

Rego, E. H.

L. Shao, P. Kner, E. H. Rego, and M. G. Gustafsson, “Super-resolution 3D microscopy of live whole cells using structured illumination,” Nat. Methods 8(12), 1044–1046 (2011).
[Crossref] [PubMed]

Rey-Suarez, I.

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

Ryu, S. W.

J. Min, J. Jang, D. Keum, S. W. Ryu, C. Choi, K. H. Jeong, and J. C. Ye, “Fluorescent microscopy beyond diffraction limits using speckle illumination and joint support recovery,” Sci. Rep. 3(1), 2075 (2013).
[Crossref] [PubMed]

Savatier, J.

E. Mudry, K. Belkebir, J. Girard, J. Savatier, E. Le Moal, C. Nicoletti, M. Allain, and A. Sentenac, “Structured illumination microscopy using unknown speckle patterns,” Nat. Photonics 6(5), 312–315 (2012).
[Crossref]

Sentenac, A.

E. Mudry, K. Belkebir, J. Girard, J. Savatier, E. Le Moal, C. Nicoletti, M. Allain, and A. Sentenac, “Structured illumination microscopy using unknown speckle patterns,” Nat. Photonics 6(5), 312–315 (2012).
[Crossref]

Shao, L.

L. Shao, P. Kner, E. H. Rego, and M. G. Gustafsson, “Super-resolution 3D microscopy of live whole cells using structured illumination,” Nat. Methods 8(12), 1044–1046 (2011).
[Crossref] [PubMed]

Shroff, H.

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

M. Ingaramo, A. G. York, P. Wawrzusin, O. Milberg, A. Hong, R. Weigert, H. Shroff, and G. H. Patterson, “Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue,” Proc. Natl. Acad. Sci. U.S.A. 111(14), 5254–5259 (2014).
[Crossref] [PubMed]

A. G. York, P. Chandris, D. D. Nogare, J. Head, P. Wawrzusin, R. S. Fischer, A. Chitnis, and H. Shroff, “Instant super-resolution imaging in live cells and embryos via analog image processing,” Nat. Methods 10(11), 1122–1126 (2013).
[Crossref] [PubMed]

A. G. York, S. H. Parekh, D. Dalle Nogare, R. S. Fischer, K. Temprine, M. Mione, A. B. Chitnis, C. A. Combs, and H. Shroff, “Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy,” Nat. Methods 9(7), 749–754 (2012).
[Crossref] [PubMed]

Song, Z.

N. Han and Z. Song, “Bayesian multiple measurement vector problem with spatial structured sparsity patterns,” Digit. Signal Process. 75, 184–201 (2018).
[Crossref]

Ströhl, F.

F. Ströhl and C. F. Kaminski, “A joint Richardson-Lucy deconvolution algorithm for the reconstruction of multifocal structured illumination microscopy data,” Methods Appl. Fluoresc. 3(1), 014002 (2015).
[Crossref] [PubMed]

Taraska, J. W.

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

Temprine, K.

A. G. York, S. H. Parekh, D. Dalle Nogare, R. S. Fischer, K. Temprine, M. Mione, A. B. Chitnis, C. A. Combs, and H. Shroff, “Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy,” Nat. Methods 9(7), 749–754 (2012).
[Crossref] [PubMed]

Torkelsen, F. H.

E. N. Ward, F. H. Torkelsen, and R. Pal, “Enhancing multi-spot structured illumination microscopy with fluorescence difference,” R. Soc. Open Sci. 5(3), 171336 (2018).
[Crossref] [PubMed]

Trexler, A. J.

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

Upadhyaya, A.

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

Vishwasrao, H. D.

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

Ward, E. N.

E. N. Ward, F. H. Torkelsen, and R. Pal, “Enhancing multi-spot structured illumination microscopy with fluorescence difference,” R. Soc. Open Sci. 5(3), 171336 (2018).
[Crossref] [PubMed]

Waterman, C. M.

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

Wawrzusin, P.

M. Ingaramo, A. G. York, P. Wawrzusin, O. Milberg, A. Hong, R. Weigert, H. Shroff, and G. H. Patterson, “Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue,” Proc. Natl. Acad. Sci. U.S.A. 111(14), 5254–5259 (2014).
[Crossref] [PubMed]

A. G. York, P. Chandris, D. D. Nogare, J. Head, P. Wawrzusin, R. S. Fischer, A. Chitnis, and H. Shroff, “Instant super-resolution imaging in live cells and embryos via analog image processing,” Nat. Methods 10(11), 1122–1126 (2013).
[Crossref] [PubMed]

Weigert, R.

M. Ingaramo, A. G. York, P. Wawrzusin, O. Milberg, A. Hong, R. Weigert, H. Shroff, and G. H. Patterson, “Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue,” Proc. Natl. Acad. Sci. U.S.A. 111(14), 5254–5259 (2014).
[Crossref] [PubMed]

Weiss, S.

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A. 106(52), 22287–22292 (2009).
[Crossref] [PubMed]

Winoto, L.

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[Crossref] [PubMed]

Wipf, D. P.

D. P. Wipf and B. D. Rao, “An empirical Bayesian strategy for solving the simultaneous sparse approximation problem,” IEEE Trans. Signal Process. 55(7), 3704–3716 (2007).
[Crossref]

Wu, J.

Wu, X.

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

Wu, Y.

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

Yang, T.

Ye, J. C.

J. Min, J. Jang, D. Keum, S. W. Ryu, C. Choi, K. H. Jeong, and J. C. Ye, “Fluorescent microscopy beyond diffraction limits using speckle illumination and joint support recovery,” Sci. Rep. 3(1), 2075 (2013).
[Crossref] [PubMed]

York, A. G.

M. Ingaramo, A. G. York, P. Wawrzusin, O. Milberg, A. Hong, R. Weigert, H. Shroff, and G. H. Patterson, “Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue,” Proc. Natl. Acad. Sci. U.S.A. 111(14), 5254–5259 (2014).
[Crossref] [PubMed]

A. G. York, P. Chandris, D. D. Nogare, J. Head, P. Wawrzusin, R. S. Fischer, A. Chitnis, and H. Shroff, “Instant super-resolution imaging in live cells and embryos via analog image processing,” Nat. Methods 10(11), 1122–1126 (2013).
[Crossref] [PubMed]

A. G. York, S. H. Parekh, D. Dalle Nogare, R. S. Fischer, K. Temprine, M. Mione, A. B. Chitnis, C. A. Combs, and H. Shroff, “Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy,” Nat. Methods 9(7), 749–754 (2012).
[Crossref] [PubMed]

Yu, B.

Zhang, S.

Appl. Opt. (1)

Digit. Signal Process. (1)

N. Han and Z. Song, “Bayesian multiple measurement vector problem with spatial structured sparsity patterns,” Digit. Signal Process. 75, 184–201 (2018).
[Crossref]

IEEE Trans. Signal Process. (1)

D. P. Wipf and B. D. Rao, “An empirical Bayesian strategy for solving the simultaneous sparse approximation problem,” IEEE Trans. Signal Process. 55(7), 3704–3716 (2007).
[Crossref]

J. Microsc. (1)

M. G. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc. 198(2), 82–87 (2000).
[Crossref] [PubMed]

Methods Appl. Fluoresc. (1)

F. Ströhl and C. F. Kaminski, “A joint Richardson-Lucy deconvolution algorithm for the reconstruction of multifocal structured illumination microscopy data,” Methods Appl. Fluoresc. 3(1), 014002 (2015).
[Crossref] [PubMed]

Nat. Methods (5)

M. Guo, P. Chandris, J. P. Giannini, A. J. Trexler, R. Fischer, J. Chen, H. D. Vishwasrao, I. Rey-Suarez, Y. Wu, X. Wu, C. M. Waterman, G. H. Patterson, A. Upadhyaya, J. W. Taraska, and H. Shroff, “Single-shot super-resolution total internal reflection fluorescence microscopy,” Nat. Methods 15(6), 425–428 (2018).
[Crossref] [PubMed]

A. G. York, P. Chandris, D. D. Nogare, J. Head, P. Wawrzusin, R. S. Fischer, A. Chitnis, and H. Shroff, “Instant super-resolution imaging in live cells and embryos via analog image processing,” Nat. Methods 10(11), 1122–1126 (2013).
[Crossref] [PubMed]

A. G. York, S. H. Parekh, D. Dalle Nogare, R. S. Fischer, K. Temprine, M. Mione, A. B. Chitnis, C. A. Combs, and H. Shroff, “Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy,” Nat. Methods 9(7), 749–754 (2012).
[Crossref] [PubMed]

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[Crossref] [PubMed]

L. Shao, P. Kner, E. H. Rego, and M. G. Gustafsson, “Super-resolution 3D microscopy of live whole cells using structured illumination,” Nat. Methods 8(12), 1044–1046 (2011).
[Crossref] [PubMed]

Nat. Photonics (1)

E. Mudry, K. Belkebir, J. Girard, J. Savatier, E. Le Moal, C. Nicoletti, M. Allain, and A. Sentenac, “Structured illumination microscopy using unknown speckle patterns,” Nat. Photonics 6(5), 312–315 (2012).
[Crossref]

Opt. Lett. (1)

Phys. Rev. Lett. (1)

C. B. Müller and J. Enderlein, “Image scanning microscopy,” Phys. Rev. Lett. 104(19), 198101 (2010).
[Crossref] [PubMed]

Proc. Natl. Acad. Sci. U.S.A. (2)

M. Ingaramo, A. G. York, P. Wawrzusin, O. Milberg, A. Hong, R. Weigert, H. Shroff, and G. H. Patterson, “Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue,” Proc. Natl. Acad. Sci. U.S.A. 111(14), 5254–5259 (2014).
[Crossref] [PubMed]

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A. 106(52), 22287–22292 (2009).
[Crossref] [PubMed]

R. Soc. Open Sci. (1)

E. N. Ward, F. H. Torkelsen, and R. Pal, “Enhancing multi-spot structured illumination microscopy with fluorescence difference,” R. Soc. Open Sci. 5(3), 171336 (2018).
[Crossref] [PubMed]

Sci. Rep. (1)

J. Min, J. Jang, D. Keum, S. W. Ryu, C. Choi, K. H. Jeong, and J. C. Ye, “Fluorescent microscopy beyond diffraction limits using speckle illumination and joint support recovery,” Sci. Rep. 3(1), 2075 (2013).
[Crossref] [PubMed]

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Figures (9)

Fig. 1
Fig. 1 Image acquisition and processing of conventional MSIM and MSIMMSBL.
Fig. 2
Fig. 2 (a) One raw image. (b) Application of digital pinholes around each PSF point. (c) Twofold contraction result of (b). (d) Superposition of all proceed raw images. (e) Deconvolution result of (d).
Fig. 3
Fig. 3 Method to transform the raw images and PSFs into matrix form.
Fig. 4
Fig. 4 Comparison of simulated MSIM and MSIMMSBL. (a) Sample with spoke-like structure. (b) Wide-field image. (c) Simulated MSIM reconstruction. (d) Simulated MSIMMSBL reconstruction. Intensity plot along the (e) green line and (f) white line indicated on images. Scale bars: 600 nm.
Fig. 5
Fig. 5 Comparison of simulated MSIM and MSIMMSBL on (a) point emitters and (b) line emitters.
Fig. 6
Fig. 6 Comparison of experiment MSIM and MSIMMSBL. (a) Wide-field image, (b) MSIM reconstruction image, and (c) MSIMMSBL reconstruction image of microtubules. (d) Intensity profile through the short white line in (a)-(c).
Fig. 7
Fig. 7 Optical configuration of multifocal structured illumination microscopy.
Fig. 8
Fig. 8 (a) Scanning pattern of DMD. (b) The fluorescence image of the excitation foci in a uniform solution of Rhodamine 6G at the sample plane.
Fig. 9
Fig. 9 Comparison of experiment MSIM and MSIMMSBL. (a) Wide-field image, (b) MSIM reconstruction image, and (c) MSIMMSBL reconstruction image of F-actin. (d) Intensity profile through the short white line in (a)-(c).

Equations (3)

Equations on this page are rendered with MathJax. Learn more.

D m (r)=PSF(r)[s(r) e m (r)],m=1,...,N.
Y=HX,
X =arg max X p(X|Y) =arg max X p(Y|X)p(X).

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