Abstract
An easy-to-use measurement method requiring no cleaning between samples, was developed and applied
for the determination of cows' plasma constituents. Whole blood and plasma samples were measured with
blood-collecting tubes as sample cells, providing easy sample handling and presentation. For whole
blood, near infrared (NIR) spectra in the short wavelength region from 600 to 1000 nm were recorded with
a portable NIR instrument (Fruit Tester 20: FT20). Plasma samples were measured in both the short
wavelength region with the FT20 and in the long wavelength region from 1100 to 2500 nm with an
NIRSystems 6500. Both instruments were equipped with specially designed sample holders for
blood-collecting tubes and fibre optics. Partial least squares (PLS) regression was applied to derive
calibrations for total protein, total cholesterol and glucose. Whole blood showed inferior performance
to plasma because of scattering interference and absorption by red blood cells. Plasma results heavily
depended on the constituent analysed. Total protein was determined accurately in both wavelength regions
due to its high concentration and high absorptivity. For total cholesterol, the long wavelength region
provided much better results because of its higher absorptivity. Results for glucose were only mediocre
in both regions as its concentration and absorptivity were low. Overall, results indicate that
blood-collecting tubes can be easily and effectively used for quantitative analysis of major
constituents in blood plasma.
© 2006 NIR Publications
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