Abstract

A personal computer-based microphotometry system is described which provides objective measurements of important diagnostic properties of histologic specimens. Characteristic features of individual nuclei such as total integrated nuclear optical density (DNA content), nuclear area, shape, and texture may be measured. When operated at reduced magnification, measurements of mitotic density, nuclear crowding, and relative nuclear orientation provide additional diagnostic information. These features may be examined in the context provided by other nuclei and, in tissues such as stratified epithelia, relative to nuclear position within the histologic structure as a whole.

© 1987 Optical Society of America

High performance pathology workstation using an automated multispectral microscope

Harvey L. Kasdan, Kenneth C. Langford, John Liberty, Michael Zachariash, and Fred H. Deindoerfer
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Image DNA-index (ploidy) analysis in cancer diagnosis

P. S. Oud, A. G. J. M. Hanselaar, M. M. M. Pahlplatz, J. W. R. Meijer, and G. Peter Vooijs
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