Chromatin texture of melanocytic nuclei: correlation between light and electron microscopy

Wolfgang Abmayr; Wilhelm Stolz; Susanne Korherr; Wolfgang Wild; Christian Schmoeckel

doi:10.1364/AO.26.003343

Applied Optics
Vol. 26,
Issue 16,
pp. 3343-3348
(1987)
•https://doi.org/10.1364/AO.26.003343

Chromatin texture of melanocytic nuclei: correlation between light and electron microscopy

Wolfgang Abmayr, Wilhelm Stolz, Susanne Korherr, Wolfgang Wild, and Christian Schmoeckel

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Wolfgang Abmayr, Wilhelm Stolz, Susanne Korherr, Wolfgang Wild, and Christian Schmoeckel, "Chromatin texture of melanocytic nuclei: correlation between light and electron microscopy," Appl. Opt. 26, 3343-3348 (1987)

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Abstract

Cells of a benign pigmented mole and a malignant melanoma were used to compare electron microscopy (EM) and light microscopy (LM) with high-resolution TV-scanning and multivariate analysis methods. Special emphasis was placed on different kinds of chromatin texture features and their discriminating power. Comparative studies on EM and LM are useful to look for the reliability of light microscopic features for the recognition of malignant melanoma cells. Identical cells of benign pigmented moles and malignant melanomas could be scanned by both EM and LM techniques. As a result, there is a significant correlation between EM and LM texture features, derived from the co-occurrence matrix and the difference statistics histogram. This helps to demonstrate that classification in LM is sufficiently good using texture features only.

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Feature	r_LE	r_{LE · A}	r_LA	r_EA
(a) 73 melanoma cells
CO-IDM	0.671	0.608	0.414	0.567
CO-ASM	0.672	0.617	0.356	0.464
CO-DI	0.629	0.531	−0.436	−0.533
CO-SAV	0.615	0.572	−0.438	−0.342
DIFF-ASM	0.672	0.616	0.425	0.501
DIFF-MEAN	0.668	0.615	−0.395	−0.510
DIFF-ENT	0.644	0.584	−0.408	−0.482
(b) 163 nevocytic nevus cells
CO-IDM	0.510	0.465	0.363	0.522
CO-ASM	0.429	0.409	0.215	0.454
CO-DI	0.528	0.472	−0.343	−0.439
CO-SAV	0.587	0.535	−0.496	−0.433
DIFF-ASM	0.591	0.521	0.444	0.468
DIFF-MEAN	0.587	0.509	−0.443	−0.495
DIFF-ENT	0.573	0.498	−0.422	−0.445

Classification matrix (EM)
Group	Percent correct	Number of nuclei classified into group
Group	Percent correct	NN	MM
NN	90.6	173	18
MM	82.5	30	141
Total	86.7	203	159
Jack-knifed classification (EM)
Group	Percent correct	Number of nuclei classified into group
Group	Percent correct	NN	MM

NN	90.6	173	18
MM	82.5	30	141
Total	86.7	203	159
Summary table (EM)
Step number	Variable entered	F value to enter or remove	% correct classification

1	19 FHP136	79.4516	65.2
2	14 FHP121	156.9410	80.4
3	18 FHP133	34.6105	86.7

Classification matrix (LM)
Group	Percent correct	Number of nuclei classified into group
Group	Percent correct	NN	MM
NN	86.0	215	35
MM	64.4	89	161
Total	75.2	304	196
Jack-knifed classification (LM)
Group	Percent correct	Number of nuclei classified into group
Group	Percent correct	NN	MM

NN	86.0	215	35
MM	64.0	90	160
Total	75.0	305	195
Summary table (LM)
Step number	Variable entered	F value to enter or remove	% correct classification

1	14 FHP121	159.9350	71.1
2	12FHP115	6.9705	72.3
3	15 FHP124	4.9563	72.8
4	17FHP130	13.5791	75.2

Feature	r_LE	r_{LE · A}	r_LA	r_EA
(a) 73 melanoma cells
CO-IDM	0.671	0.608	0.414	0.567
CO-ASM	0.672	0.617	0.356	0.464
CO-DI	0.629	0.531	−0.436	−0.533
CO-SAV	0.615	0.572	−0.438	−0.342
DIFF-ASM	0.672	0.616	0.425	0.501
DIFF-MEAN	0.668	0.615	−0.395	−0.510
DIFF-ENT	0.644	0.584	−0.408	−0.482
(b) 163 nevocytic nevus cells
CO-IDM	0.510	0.465	0.363	0.522
CO-ASM	0.429	0.409	0.215	0.454
CO-DI	0.528	0.472	−0.343	−0.439
CO-SAV	0.587	0.535	−0.496	−0.433
DIFF-ASM	0.591	0.521	0.444	0.468
DIFF-MEAN	0.587	0.509	−0.443	−0.495
DIFF-ENT	0.573	0.498	−0.422	−0.445

Classification matrix (EM)
Group	Percent correct	Number of nuclei classified into group
Group	Percent correct	NN	MM
NN	90.6	173	18
MM	82.5	30	141
Total	86.7	203	159
Jack-knifed classification (EM)
Group	Percent correct	Number of nuclei classified into group
Group	Percent correct	NN	MM

NN	90.6	173	18
MM	82.5	30	141
Total	86.7	203	159
Summary table (EM)
Step number	Variable entered	F value to enter or remove	% correct classification

1	19 FHP136	79.4516	65.2
2	14 FHP121	156.9410	80.4
3	18 FHP133	34.6105	86.7

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Applied Optics