20 February 2023, Volume 10, Issue 2, pp. 142-307

By combining multiplane or region-of-interest illumination, and spherical-aberration-assisted point spread function in detection space, SImultaneous MultiPLanE imaging light-sheet fluorescence microscopy (SIMPLE-LSFM) can simultaneously acquire different depths of a sample. The SIMPLE light-sheet methods enable high-speed cellular-resolution multi-depth-resolved mapping of anatomic structure and fast dynamics in three dimensions. See Jiao et al., pp. 239–247. See print version of the cover.