Abstract

In this Letter, we present, to the best of our knowledge, a novel illumination modulation method for reflective and fluorescent separation by using only one spectral image. Specifically, we present an optical system using off-the-shelf devices to generate high frequency illumination, which is desirable in reflective-fluorescent separation tasks. In addition, we employ the total variation regularization scheme to account for spectral–spatial correlation, which makes our method robust to noise. Experiments on both simulated and real data verify the effectiveness and practicality of our method.

© 2020 Optical Society of America

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