Abstract
Structured illumination microscopy (SIM) is a powerful technique for providing super-resolution imaging, but its reconstruction algorithm, i.e., linear reconstruction structured illumination microscopy (LRSIM) algorithm in the Fourier domain, limits the imaging speed due to its computational effort. Here, we present a novel reconstruction algorithm that can directly process SIM data in the spatial domain. Compared to LRSIM, this approach uses the same number of frames to achieve a comparable resolution but with a much faster processing speed. Our algorithm was verified on both simulated and experimental data using sinusoidal pattern illumination. Moreover, this algorithm is also applicable for speckle pattern illumination.
© 2020 Optical Society of America
Full Article | PDF ArticleMore Like This
Shijie Tu, Xiaoyan Li, Yueying Wang, Wenwen Gong, Xin Liu, Q. Liu, Yubing Han, Cuifang Kuang, Xu Liu, and Xiang Hao
Opt. Lett. 48(10) 2535-2538 (2023)
Florian Ströhl and Clemens F. Kaminski
Opt. Lett. 42(13) 2511-2514 (2017)
Xingye Chen, Karl Zhanghao, Meiqi Li, Chang Qiao, Wenhui Liu, Peng Xi, and Qionghai Dai
Opt. Express 28(17) 25642-25654 (2020)