Abstract

The changes of mechanical properties and biological activities of monomeric erythrocytes are studied using optical tweezers micromanipulation technology. Firstly, the mechanical properties of irradiated erythrocyte membranes are obtained. Weaker power laser irradiation can delay the decay of the mechanical properties of erythrocytes and promote the biological activity of erythrocytes, while higher power laser irradiation damages erythrocytes. The stronger the laser irradiation is, the more obvious and rapid the damage will be. The temperature of the cell surface will be changed by regulating the laser power and irradiation time, so the biological functions of erythrocyte can be controlled. Secondly, the finite element simulation of the temperature change on the cell surface under the condition of laser irradiation is carried out using simulation software, and the precise temperature of the cell surface irradiated cumulatively by a laser with different powers is obtained. Finally, the processes of abscission, unfolding, and denaturation of hemoglobins in erythrocytes at different temperatures due to the photothermal effect are analyzed using the model. The mechanism of laser irradiation on the elasticity of erythrocyte membranes is also obtained.

© 2019 Chinese Laser Press

Study of in vitro RBCs membrane elasticity with AOD scanning optical tweezers

Huadong Song, Ying Liu, Bin Zhang, Kangzhen Tian, Panpan Zhu, Hao Lu, and Qi Tang
Biomed. Opt. Express 8(1) 384-394 (2017)

High throughput trapping and arrangement of biological cells using self-assembled optical tweezer

Zongbao Li, Jianxin Yang, Shaojing Liu, Xiaofang Jiang, Haiyan Wang, Xiaowen Hu, Sheng Xue, Sailing He, and Xiaobo Xing
Opt. Express 26(26) 34665-34674 (2018)

Light distribution in the erythrocyte under laser irradiation: a finite-difference time-domain calculation

Xiao-Qian Wang, Ji-Tong Yu, Pei-Nan Wang, and Ji-Yao Chen
Appl. Opt. 47(22) 4037-4044 (2008)

Analysis of the behaviour of erythrocytes in an optical trapping system

S.C. Grover, R.C. Gauthier, and A.G. Skirtach
Opt. Express 7(13) 533-539 (2000)

Measurement of red blood cell deformability during morphological changes using rotating-glass-plate-based scanning optical tweezers

Rui Liu, Meng Shao, Zeyu Ke, Changxu Li, Fengya Lu, Min-Cheng Zhong, Yuxin Mao, Xunbin Wei, Zhensheng Zhong, and Jinhua Zhou
Biomed. Opt. Express 14(9) 4979-4989 (2023)