Abstract
This letter presents a label-free biomolecular imaging technique based on white-light
interferometry and spectral detection. The method measures thickness changes caused by specific binding
between biomolecules to detect the presence of certain analyte. A spectrum-shifting algorithm is developed to
resolve the thickness information from the spectrum. The axial resolution of the experimental instrument can
reach ~1 nm, thereby enabling detection of trace amounts (~1 ng/mm2) of proteins or DNA. This letter also
presents two experiments to prove the feasibility of the method for detecting proteins and DNA without
fluorescent labeling.
© 2013 Chinese Optics Letters
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