Abstract

Morphological assessments are used to select embryos with the highest implantation potential, however it is still very limited. The development of new technologies, such as Raman spectroscopy have improved quantitative and qualitative analysis, and consequently led to a better characterization of embryos and improvements on the prediction of their potential. Therefore, we propose a method based on the conventional in vitro culture system of bovine embryos, and the subsequent analysis of the culture media drops by Raman spectroscopy. Our results obtained by PCA analysis clearly showed a separation of the spectral profiles from culture media drops with and without embryos.

© 2015 Optical Society of America

1. Introduction

The main goal of in vitro production (IVP) of bovine embryos is increase pregnancy rates from IVP embryos transferred to the recipients, resulting in several economical and social benefits to cattle producers [1]. To reach this goal, researchers seek for improvements in the embryo selection criteria, aiming to transfer the best embryos to recipients, with high development potential and pregnancy success [2, 3].

Several methods have been proposed for the evaluation of embryo quality, such as assessment of eclosion rates, survival to cryopreservation, cell count of the inner cell mass and trophoectoderm, evaluation of the number of apoptotic cells, and embryo biopsy for chromosomal abnormalities analysis. However, many of these techniques are not feasible for practical application in IVP, since they can compromise the embryo viability and prevent its transfer to recipients [1]. Thus, bovine embryos selection for embryo transfer (ET) is based on morphology and embryonic stage of development, but these criteria are not good predictors of embryo survival [4–6].

Therefore, new methods has been sought - preferably non-invasive ones - to provide more accurate information that can be correlated to the quality and development potential of bovine IVP embryos [7, 8]. Among non-invasive techniques, the evaluation of embryo culture medium is a new approach that has been gaining attention recently, since it is the direct microenvironment of the IVP embryo [9, 10]. Studies suggest that the detection of chemical composition changes in the culture medium can be correlated with the embryo quality and development potential [3, 11–13].

Several technologies have been used to evaluate culture media, such as Nuclear Magnetic Resonance (NMR) [14], Mass Spectrometry (MS) [15], Fourier Transform Infrared Spectroscopy (FT-IR) [1], Near-infrared Spectroscopy (NIR) [16], Raman Spectroscopy [10], among others.

The Raman spectroscopy technique has special interest due to their high sensitivity to detect tiny biochemical and molecular variations in tissues or biological samples [17]. The characteristic spectra profiles generated by this technique can be applied as a non destructive analytical method. Other advantages are related to the small sample volume required (only some µL) and the minimal previous preparation needed [10, 18]. Therefore, Raman spectroscopy can be considered as a very promising technique for the evaluation of embryo culture media.

Using this technique, some authors have demonstrated the presence of different spectra profiles of IVP culture media for embryos with good development and implantation potential in comparison with ones that resulted in implantation failure after transfer [10, 16, 18]. It’s important to mention that the evaluation of embryo culture media by Raman spectroscopy has been performed in humans, but the same is not true for other species, such as bovine. Therefore, the objective of this study was the development and standardization of a method based on Raman micro-spectroscopy for the evaluation of the culture media of IVP bovine embryos.

2. Materials and methods

All procedures and protocols were performed in accordance with the Ethical Principles in Animal Research set forth by the Brazilian College of Animal Experimentation, with approval from “Ethic Committee in the use of animal” of Universidade Federal do ABC (Protocol No. 008/2014).

2.1 Sample preparation

Embryos were produced following standard protocols for in vitro maturation (IVM) and fertilization (IVF) [19]. During the in vitro culture (IVC) the zygotes were transferred individually into microdroplets of 20μl of culture medium and the individual cultures were placed in an incubator at 38.5°C and 5% CO2 in air and high humidity for seven days.

For evaluation by Raman spectroscopy three standard culture media for the IVC of the embryos were used: (i) Potassium Simplex Optimization Medium (KSOM) (MR-106-D Millipore) supplemented with 10% of fetal bovine serum (FBS), gentamicin and non-essential amino acids; (ii) Synthetic Oviduct Fluid (SOF) supplemented with 5% FBS, essential and non-essential amino acids (SOFaa); (iii) SOFaa collected after seven days of embryo in vitro culture (SOFaaE). All samples remained in an incubator at 38.5°C and 5% CO2 in air and high humidity for seven days before being collected and stored at −80°C until analysis.

2.2 Raman spectroscopy

The equipment used on this study was the Triple T64000 Raman Spectrometer (Horiba Jobin-Yvon S.A.S., France) with microanalysis option and CCD detector 1024x256 – OPEN-3LD/R with quantum response of approximately 40%. The excitation laser was a 532 nm (Verdi G5, Coherent Inc. USA) focused on a spot with 5 mW power. Spectra were acquired from four biological replicates. Each replicate was composed by five embryos at blastocyst stage. One spectrum was collected per droplet.

2.3 Spectra acquisition

During the standardization of the method parameters such as droplet volume, presence or not of mineral oil, kind of substrate, objective magnification, degree of confocability, and acquisition time/number of accumulations were tested. Figure 1 shows a schematic diagram of the experiment using Petri dish as substrate and microdrops culture media covered with mineral oil (Fig. 1(A)). That set was placed in the upright micro-Raman microscope. The laser was focused through the mineral oil layer (Fig. 1(B)).

 

Fig. 1 Schematic diagram of the proposed experimental model. A) culture medium droplets covered with mineral oil on Petri dish in the micro-Raman system; B) incidence of the laser beam on the sample with the spectra acquisition.

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The characterization of the culture medium droplets was performed using the optimized parameters found. It was used a confocal analysis with the spectra being acquired from the mineral oil surface and at every 2 µm interval in the z-axis (Fig. 1(A)) using the microscope micrometer.

2.4 Spectra analysis

Experimental data were plotted using the Origin 8.0 software (OriginLab, Northampton, MA). Spectra were acquired by Labscap 5.0 software and pre-processed by removing spikes. The baseline correction and normalization by area of the spectra was performed by Fityk 0.9.8 software [20]. Spectra and loading plot were analyzed by Principal Component Analysis (PCA), subroutines of the software Minitab 16 (Minitab Inc.). Vector normalization was made for the analysis done by PCA, and PCs were obtained from a correlation matrix.

3. Results and discussion

3.1 Standardization of spectra parameters

The choice of the substrate was the first step of the standardization procedure. It was tested Petri dish (polystyrene), commercial aluminum foil, CaF2 window and glass slides. A chemically stable substrate that does not interfere in the spectra acquisition of the samples, such as quartz [16, 18] and gold [9, 10] is the usual option of choice for Raman experiments in culture media. Nevertheless, the selected substrate for this experiment was the Petri dish. Others substrates generated contamination with spurious Raman bands. Moreover, Petri dish is a widely employed culture cell consumable which enables evaluation of the proposed methodology in a closer IVP lab scenario.

As for the sample preparation, volumes of 10 and 20 µL of culture medium, with and without a cover of mineral oil on the polystyrene Petri dish, were tested. The droplet with 10 µL of culture medium covered with mineral oil on the Petri dish resulted in the best configuration for spectra acquisition and applicability of the proposed method. The use of a smallest volume of culture medium is essential because of the limited availability of material derived from embryo culture. The choice to scan the samples covered with mineral oil was made because it is also the method used for in vitro culture [19, 21] increasing, therefore, the commercial application of this method. Furthermore, the tests carried out without the use of mineral oil covering the culture medium droplets, showed that the incidence of the laser beam, even with low power, caused sample evaporation during the spectra acquisition. This reduction of the sample volume could generate erroneous data, especially in a quantitative analysis, and also prevent the reproducibility of the results [17].

Regarding the use of objective lenses for spectral acquisition, tests were conducted using 10, 50, and 100X magnification objectives. At long working distance (10.6 µm), the plan achromatic 50X objective (NA = 0.50) showed better results, with best signal-to-noise ratio. The confocability of the system was also tested, ranging the confocal aperture in 1, 3, 5 and 6.5 µm. The best results were observed with the 6.5 µm confocal aperture which presented spectra with best signal-to-noise ratio and higher bands intensity.

Moreover tests were conducted varying the spectral range to analyze the samples: 500 to 1800 cm−1, 1800 to 4000 cm−1, 500 to 3200 cm−1 and 500 to 4000 cm−1. The spectra were acquired on the 500 - 3200 cm−1 spectral window. It is also a region that contains bands with biological relevance (see Table 1), such as proteins, amino acids, lipids, among other bands [9, 10, 16–18]. Finally, tests were also made by varying acquisition number x time of exposure: 1 x 10 s, 2 x 30 s, 3 x 20 s. The best acquisition number x time of exposure was 3 x 20 s since it produced bands with higher signal intensity.

Tables Icon

Table 1. Raman bands assignments of the culture medium with embryos (SOFaaE).

3.2 Confocal analysis of the droplet

The opening of the confocal aperture was also optimized. The best value was 6.5 µm. In order to identify the oil-culture interface spectra were acquired for several z-values (Fig. 2). It was possible to identify three distinct regions. The first consists of the spectra collected in the oil surface (0 µm) to 14 µm z-axis displacement, which it is noted that the spectra show fairly constant relative intensity of the bands. The second region occurs between 16 and 18 µm z-axis, comprises the interface between the mineral oil and the culture medium, evidenced by the slight increase in the intensity of the bands. The third region is from 20 µm of z axis, shows the spectra collected from the culture medium itself, evidenced by a gradual increase in the intensity of the bands.

 

Fig. 2 Oil-culture interface identification in the droplet. The two highlighted regions corresponding to the spectrum of the surface of the mineral oil (0 µm in the z-axis) and the interface between oil and culture medium (18 µm z-axis) are shown.

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It was determined z = 20 µm the suitable depth for culture medium data acquisition. It is important to notice that the distinct droplet regions presented almost the same set of Raman bands. Only an overall intensity variation was observed. It is well-reported that some culture medium molecules, as lipids, could be absorbed by the mineral oil [9, 21, 22] which justify this finding.

It was described a method for culture media analysis based on the deposition and drying of the droplet on a solid substrate [17]. The pre-concentration of the sample constituents on the substrate allows their capture with a high intensity signal in the Raman spectrum. However, the droplet drying technique results in a coffee ring effect, in which proteins precipitate and accumulate in the peripheral region of the ring, providing a Raman spectrum with high intensity signal. The central region, though, contains a large precipitation of salts, mainly NaCl and KCl, with low intensity signal. Thus, the method proposed in this study, with no drying up of the droplet, has the advantage of faithfully representing the composition of the culture medium.

3.3 Sample analysis

Raman spectra were acquired from the substrate (Petri dish), mineral oil and the culture media with and without embryos. Figure 3 shows the spectra of the culture media without embryos (KSOM and SOFaa), with embryos (SOfaaE), Petri dish and mineral oil. It was evident that the substrate showed a very distinct and characteristic spectrum in comparison with the culture media spectra, indicating that it didn’t interfere in the culture media analysis. Some of the mineral oil bands coincided with those from the culture media, however they had distinct intensities. The presence of coincident bands between these samples could be caused by the moving of culture medium molecules to the oil during the embryo culture [21–23]. It is also important to note that mineral oil is a product derived from crude oil, and therefore, has a chemical composition variable in quality [21], which can result in various bands in the Raman spectra.

 

Fig. 3 Average Raman spectra of the following groups: culture media without embryos (KSOM and SOFaa), with embryos (SOFaaE), mineral oil and Petri dish in the 500-3200 cm−1 region.

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The analysis of embryo culture medium (SOFaaE) showed vibrational modes of important biomolecules, especially large classes of proteins, lipids and nucleic acids, as expected [9, 10, 17]. Figure 4 shows the Raman spectra of SOFaaE with the corresponding bands, which are identified in Table 1.

 

Fig. 4 Raman spectra of culture medium with embryos (SOFaaE) with their corresponding vibrational bands in the 500 to 3200 cm−1 region.

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The technique sensitivity was evaluated from the PCA analysis of the culture media spectra without embryos (KSOM, SOFaa) and with embryos (SOFaaE). Since the spectra scan was set on a broad region, the PCA analysis was separated in two ranges, one from 500 to 1800 cm−1 (fingerprint) and the other from 1800 to 3200 cm−1 (high wavenumber). The percentage of variance for each PC was PC1: 98.4%; PC2: 1.5%; PC3: 0.1%; PC4 < 0.1% for fingerprint region. Similar values were found for high wavenumber region. Due to the negligible contribution from PCs greater than 4 they were not considered in the analysis. Figure 5 presents the dispersion curves for PC1, PC2, and PC3. It was possible to see a clear separation between the spectroscopic profiles of the three groups analyzed in both ranges evaluated. This separation shows the sensitivity of the technique to generate specific and characteristic spectroscopic profiles of the culture media with and without embryos.

 

Fig. 5 Principal Component Analysis (PCA) of the Raman spectra of the culture media without embryos (KSOM and SOFaa) and with embryos (SOFaaE) in the Raman bands from 500 to 1800 cm−1 (A) and from 1800 to 3200 cm−1 (B).

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Loading plot analysis from PCs responsible for the separation of the groups are shown in Fig. 6. Bands that contribute in a higher proportion to the separation of the groups were identified: 1442 and 1455 cm−1 (lipids and proteins bands) in 500 to 1800 cm−1 region, and 2892 cm−1 (poly methylene chain band) in 1800 to 3200 cm−1 region.

 

Fig. 6 Loading plot analysis of the Raman spectra of the culture media without embryos (KSOM and SOFaa) and with embryos (SOFaaE) in the Raman bands from 500 to 1800 cm−1 (A) and from 1800 to 3200 cm−1 (B).

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Considering the results of the method proposed in this study (summarized in Fig. 1), it’s possible to state that it is efficient for the evaluation of culture media used in IVP bovine embryos. This technique can be used as a fast and accurate diagnostic method that allow us to characterize the bovine embryo culture media in order to find global standards and characteristic profiles. The use of this profiles combined with embryo morphology data can allow a better evaluation of embryo quality, which can lead to an improvement in embryo selection and an increase in pregnancy rates.

4. Conclusion

This study proposes, for the first time, a method for the evaluation of culture media of in vitro produced bovine embryos based on Raman spectroscopy. The proposed method has potential for commercial application and a series of advantages such as: the equipment is relatively easy to use and handle and it is not expensive; no high cost analysis and results can be obtained immediately; use of small sample volume with no need of previous preparation or processing of the samples; and it is a non-invasive and non-destructive technique. Therefore, this technology has potential as a diagnostic application for global characterization of spectroscopic profiles of culture media used in IVP bovine embryos, which can be correlated with viability in order to improve embryo selection and consequently the pregnancy rates.

Acknowledgments

The authors would like to thank the Brazilian agencies FAPESP, CAPES, and CNPq for financial support; Multiusers Central Facilities of UFABC for the experimental support.

References and links

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2. A. S. Lopes, T. Greve, and H. Callesen, “Quantification of embryo quality by respirometry,” Theriogenology 67(1), 21–31 (2007). [CrossRef]   [PubMed]  

3. E. Seli, L. Botros, D. Sakkas, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using proton nuclear magnetic resonance correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 90(6), 2183–2189 (2008). [CrossRef]   [PubMed]  

4. D. R. Brison, F. D. Houghton, D. Falconer, S. A. Roberts, J. Hawkhead, P. G. Humpherson, B. A. Lieberman, and H. J. Leese, “Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover,” Hum. Reprod. 19(10), 2319–2324 (2004). [CrossRef]   [PubMed]  

5. C. G. Vergouw, L. L. Botros, P. Roos, J. W. Lens, R. Schats, P. G. A. Hompes, D. H. Burns, and C. B. Lambalk, “Metabolomic profiling by near-infrared spectroscopy as a tool to assess embryo viability: a novel, non-invasive method for embryo selection,” Hum. Reprod. 23(7), 1499–1504 (2008). [CrossRef]   [PubMed]  

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9. A. G. Shen, J. Peng, Q. H. Zhao, L. Su, X. H. Wang, J. M. Hu, and Q. Yang, “Accurate and noninvasive embryos screening during in vitro fertilization (IVF) assisted by Raman analysis of embryos culture medium,” Laser Phys. Lett. 9(4), 322–328 (2012). [CrossRef]  

10. Q. Zhao, T. Yin, J. Peng, Y. Zou, J. Yang, A. Shen, and J. Hu, “Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF),” Int. J. Mol. Sci. 14(4), 6556–6570 (2013). [CrossRef]   [PubMed]  

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17. Z. Huang, Y. Sun, J. Wang, S. Du, Y. Li, J. Lin, S. Feng, J. Lei, H. Lin, R. Chen, and H. Zeng, “Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy,” J. Biomed. Opt. 18(12), 127003 (2013). [CrossRef]   [PubMed]  

18. R. Scott, E. Seli, K. Miller, D. Sakkas, K. Scott, and D. H. Burns, “Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study,” Fertil. Steril. 90(1), 77–83 (2008). [CrossRef]   [PubMed]  

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References

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  1. M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).
  2. A. S. Lopes, T. Greve, and H. Callesen, “Quantification of embryo quality by respirometry,” Theriogenology 67(1), 21–31 (2007).
    [Crossref] [PubMed]
  3. E. Seli, L. Botros, D. Sakkas, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using proton nuclear magnetic resonance correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 90(6), 2183–2189 (2008).
    [Crossref] [PubMed]
  4. D. R. Brison, F. D. Houghton, D. Falconer, S. A. Roberts, J. Hawkhead, P. G. Humpherson, B. A. Lieberman, and H. J. Leese, “Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover,” Hum. Reprod. 19(10), 2319–2324 (2004).
    [Crossref] [PubMed]
  5. C. G. Vergouw, L. L. Botros, P. Roos, J. W. Lens, R. Schats, P. G. A. Hompes, D. H. Burns, and C. B. Lambalk, “Metabolomic profiling by near-infrared spectroscopy as a tool to assess embryo viability: a novel, non-invasive method for embryo selection,” Hum. Reprod. 23(7), 1499–1504 (2008).
    [Crossref] [PubMed]
  6. S. M. Pudakalakatti, S. Uppangala, F. D’Souza, G. Kalthur, P. Kumar, S. K. Adiga, and H. S. Atreya, “NMR studies of preimplantation embryo metabolism in human assisted reproductive techniques: a new biomarker for assessment of embryo implantation potential,” NMR Biomed. 26(1), 20–27 (2013).
    [Crossref] [PubMed]
  7. J. Herrero and M. Meseguer, “Selection of high potential embryos using time-lapse imaging: the era of morphokinetics,” Fertil. Steril. 99(4), 1030–1034 (2013).
    [Crossref] [PubMed]
  8. D. K. Gardner, M. Lane, J. Stevens, and W. B. Schoolcraft, “Noninvasive assessment of human embryo nutrient consumption as a measure of developmental potential,” Fertil. Steril. 76(6), 1175–1180 (2001).
    [Crossref] [PubMed]
  9. A. G. Shen, J. Peng, Q. H. Zhao, L. Su, X. H. Wang, J. M. Hu, and Q. Yang, “Accurate and noninvasive embryos screening during in vitro fertilization (IVF) assisted by Raman analysis of embryos culture medium,” Laser Phys. Lett. 9(4), 322–328 (2012).
    [Crossref]
  10. Q. Zhao, T. Yin, J. Peng, Y. Zou, J. Yang, A. Shen, and J. Hu, “Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF),” Int. J. Mol. Sci. 14(4), 6556–6570 (2013).
    [Crossref] [PubMed]
  11. D. K. Gardner and H. J. Leese, “Assessment of embryo viability prior to transfer by the noninvasive measurement of glucose uptake,” J. Exp. Zool. 242(1), 103–105 (1987).
    [Crossref] [PubMed]
  12. T. Hardarson, A. Ahlström, L. Rogberg, L. Botros, T. Hillensjö, G. Westlander, D. Sakkas, and M. Wikland, “Non-invasive metabolomic profiling of Day 2 and 5 embryo culture medium: a prospective randomized trial,” Hum. Reprod. 27(1), 89–96 (2012).
    [Crossref] [PubMed]
  13. Y. S. Lee, G. A. Thouas, and D. K. Gardner, “Developmental kinetics of cleavage stage mouse embryos are related to their subsequent carbohydrate and amino acid utilization at the blastocyst stage,” Hum. Reprod. 30(3), 543–552 (2015).
    [Crossref] [PubMed]
  14. M. Wallace, E. Cottell, J. Cullinane, F. M. McAuliffe, M. Wingfield, and L. Brennan, “(1)H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential,” Syst. Biol. Reprod. Med. 60(1), 58–63 (2014).
    [Crossref] [PubMed]
  15. S. S. Cortezzi, E. C. Cabral, M. G. Trevisan, C. R. Ferreira, A. S. Setti, D. P. Braga, R. C. Figueira, A. Iaconelli, M. N. Eberlin, and E. Borges., “Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium,” Reproduction 145(5), 453–462 (2013).
    [Crossref] [PubMed]
  16. E. Seli, D. Sakkas, R. Scott, S. C. Kwok, S. M. Rosendahl, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using Raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 88(5), 1350–1357 (2007).
    [Crossref] [PubMed]
  17. Z. Huang, Y. Sun, J. Wang, S. Du, Y. Li, J. Lin, S. Feng, J. Lei, H. Lin, R. Chen, and H. Zeng, “Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy,” J. Biomed. Opt. 18(12), 127003 (2013).
    [Crossref] [PubMed]
  18. R. Scott, E. Seli, K. Miller, D. Sakkas, K. Scott, and D. H. Burns, “Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study,” Fertil. Steril. 90(1), 77–83 (2008).
    [Crossref] [PubMed]
  19. C. A. Soares, K. Annes, T. R. Dreyer, T. Magrini, M. T. Sonoda, H. da Silva Martinho, M. Nichi, M. E. d’Ávila Assumpção, and M. P. Milazzotto, “Photobiological effect of low-level laser irradiation in bovine embryo production system,” J. Biomed. Opt. 19(3), 035006 (2014).
    [Crossref] [PubMed]
  20. M. Wojdyr, “Fityk: a general-purpose peak fitting program,” J. Appl. Cryst. 43(5), 1126–1128 (2010).
    [Crossref]
  21. C. A. Martinez, A. Nohalez, C. Cuello, J. M. Vazquez, J. Roca, E. A. Martinez, and M. A. Gil, “The use of mineral oil during in vitro maturation, fertilization, and embryo culture does not impair the developmental competence of pig oocytes,” Theriogenology 83(4), 693–702 (2015).
    [Crossref] [PubMed]
  22. M. Shimada, N. Kawano, and T. Terada, “Delay of nuclear maturation and reduction in developmental competence of pig oocytes after mineral oil overlay of in vitro maturation media,” Reproduction 124(4), 557–564 (2002).
    [Crossref] [PubMed]
  23. P. B. D. Gonçalves, M. A. L. Oliveira, A. Mezzalira, M. M. Montagner, J. A. Visitin, and L. F. S. Costa, Biotécnicas aplicadas à reprodução animal (Roca, 2008).
  24. M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Yan So, and Z. Huang, “In vivo diagnosis of gastric cancer using Raman endoscopy and ant colony optimization techniques,” Int. J. Cancer 128(11), 2673–2680 (2011).
    [Crossref] [PubMed]
  25. A. Barth and C. Zscherp, “What vibrations tell us about proteins,” Q. Rev. Biophys. 35(4), 369–430 (2002).
    [Crossref] [PubMed]
  26. S. Cinta Pinzaru, A. Falamas, and C. A. Dehelean, “Molecular conformation changes along the malignancy revealed by optical nanosensors,” J. Cell. Mol. Med. 17(2), 277–286 (2013).
    [Crossref] [PubMed]
  27. Y. Zhou, C. H. Liu, Y. Sun, Y. Pu, S. Boydston-White, Y. Liu, and R. R. Alfano, “Human brain cancer studied by resonance Raman spectroscopy,” J. Biomed. Opt. 17(11), 116021 (2012).
    [Crossref] [PubMed]
  28. O. Samek, A. Jonáš, Z. Pilát, P. Zemánek, L. Nedbal, J. Tříska, P. Kotas, and M. Trtílek, “Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo,” Sensors (Basel) 10(9), 8635–8651 (2010).
    [Crossref] [PubMed]
  29. L. Xue, P. Sun, D. Ou, P. Chen, M. Chen, and B. Yan, “Diagnosis of pathological minor salivary glands in primary Sjogren’s syndrome by using Raman spectroscopy,” Lasers Med. Sci. 29(2), 723–728 (2014).
    [Crossref] [PubMed]
  30. A. Moshaverinia, S. Ansari, Z. Movasaghi, R. W. Billington, J. A. Darr, and I. U. Rehman, “Modification of conventional glass-ionomer cements with N-vinylpyrrolidone containing polyacids, nano-hydroxy and fluoroapatite to improve mechanical properties,” Dent. Mater. 24(10), 1381–1390 (2008).
    [Crossref] [PubMed]

2015 (2)

Y. S. Lee, G. A. Thouas, and D. K. Gardner, “Developmental kinetics of cleavage stage mouse embryos are related to their subsequent carbohydrate and amino acid utilization at the blastocyst stage,” Hum. Reprod. 30(3), 543–552 (2015).
[Crossref] [PubMed]

C. A. Martinez, A. Nohalez, C. Cuello, J. M. Vazquez, J. Roca, E. A. Martinez, and M. A. Gil, “The use of mineral oil during in vitro maturation, fertilization, and embryo culture does not impair the developmental competence of pig oocytes,” Theriogenology 83(4), 693–702 (2015).
[Crossref] [PubMed]

2014 (4)

C. A. Soares, K. Annes, T. R. Dreyer, T. Magrini, M. T. Sonoda, H. da Silva Martinho, M. Nichi, M. E. d’Ávila Assumpção, and M. P. Milazzotto, “Photobiological effect of low-level laser irradiation in bovine embryo production system,” J. Biomed. Opt. 19(3), 035006 (2014).
[Crossref] [PubMed]

L. Xue, P. Sun, D. Ou, P. Chen, M. Chen, and B. Yan, “Diagnosis of pathological minor salivary glands in primary Sjogren’s syndrome by using Raman spectroscopy,” Lasers Med. Sci. 29(2), 723–728 (2014).
[Crossref] [PubMed]

M. Wallace, E. Cottell, J. Cullinane, F. M. McAuliffe, M. Wingfield, and L. Brennan, “(1)H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential,” Syst. Biol. Reprod. Med. 60(1), 58–63 (2014).
[Crossref] [PubMed]

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

2013 (6)

S. M. Pudakalakatti, S. Uppangala, F. D’Souza, G. Kalthur, P. Kumar, S. K. Adiga, and H. S. Atreya, “NMR studies of preimplantation embryo metabolism in human assisted reproductive techniques: a new biomarker for assessment of embryo implantation potential,” NMR Biomed. 26(1), 20–27 (2013).
[Crossref] [PubMed]

J. Herrero and M. Meseguer, “Selection of high potential embryos using time-lapse imaging: the era of morphokinetics,” Fertil. Steril. 99(4), 1030–1034 (2013).
[Crossref] [PubMed]

S. S. Cortezzi, E. C. Cabral, M. G. Trevisan, C. R. Ferreira, A. S. Setti, D. P. Braga, R. C. Figueira, A. Iaconelli, M. N. Eberlin, and E. Borges., “Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium,” Reproduction 145(5), 453–462 (2013).
[Crossref] [PubMed]

Q. Zhao, T. Yin, J. Peng, Y. Zou, J. Yang, A. Shen, and J. Hu, “Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF),” Int. J. Mol. Sci. 14(4), 6556–6570 (2013).
[Crossref] [PubMed]

Z. Huang, Y. Sun, J. Wang, S. Du, Y. Li, J. Lin, S. Feng, J. Lei, H. Lin, R. Chen, and H. Zeng, “Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy,” J. Biomed. Opt. 18(12), 127003 (2013).
[Crossref] [PubMed]

S. Cinta Pinzaru, A. Falamas, and C. A. Dehelean, “Molecular conformation changes along the malignancy revealed by optical nanosensors,” J. Cell. Mol. Med. 17(2), 277–286 (2013).
[Crossref] [PubMed]

2012 (3)

Y. Zhou, C. H. Liu, Y. Sun, Y. Pu, S. Boydston-White, Y. Liu, and R. R. Alfano, “Human brain cancer studied by resonance Raman spectroscopy,” J. Biomed. Opt. 17(11), 116021 (2012).
[Crossref] [PubMed]

T. Hardarson, A. Ahlström, L. Rogberg, L. Botros, T. Hillensjö, G. Westlander, D. Sakkas, and M. Wikland, “Non-invasive metabolomic profiling of Day 2 and 5 embryo culture medium: a prospective randomized trial,” Hum. Reprod. 27(1), 89–96 (2012).
[Crossref] [PubMed]

A. G. Shen, J. Peng, Q. H. Zhao, L. Su, X. H. Wang, J. M. Hu, and Q. Yang, “Accurate and noninvasive embryos screening during in vitro fertilization (IVF) assisted by Raman analysis of embryos culture medium,” Laser Phys. Lett. 9(4), 322–328 (2012).
[Crossref]

2011 (1)

M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Yan So, and Z. Huang, “In vivo diagnosis of gastric cancer using Raman endoscopy and ant colony optimization techniques,” Int. J. Cancer 128(11), 2673–2680 (2011).
[Crossref] [PubMed]

2010 (2)

O. Samek, A. Jonáš, Z. Pilát, P. Zemánek, L. Nedbal, J. Tříska, P. Kotas, and M. Trtílek, “Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo,” Sensors (Basel) 10(9), 8635–8651 (2010).
[Crossref] [PubMed]

M. Wojdyr, “Fityk: a general-purpose peak fitting program,” J. Appl. Cryst. 43(5), 1126–1128 (2010).
[Crossref]

2008 (4)

A. Moshaverinia, S. Ansari, Z. Movasaghi, R. W. Billington, J. A. Darr, and I. U. Rehman, “Modification of conventional glass-ionomer cements with N-vinylpyrrolidone containing polyacids, nano-hydroxy and fluoroapatite to improve mechanical properties,” Dent. Mater. 24(10), 1381–1390 (2008).
[Crossref] [PubMed]

C. G. Vergouw, L. L. Botros, P. Roos, J. W. Lens, R. Schats, P. G. A. Hompes, D. H. Burns, and C. B. Lambalk, “Metabolomic profiling by near-infrared spectroscopy as a tool to assess embryo viability: a novel, non-invasive method for embryo selection,” Hum. Reprod. 23(7), 1499–1504 (2008).
[Crossref] [PubMed]

E. Seli, L. Botros, D. Sakkas, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using proton nuclear magnetic resonance correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 90(6), 2183–2189 (2008).
[Crossref] [PubMed]

R. Scott, E. Seli, K. Miller, D. Sakkas, K. Scott, and D. H. Burns, “Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study,” Fertil. Steril. 90(1), 77–83 (2008).
[Crossref] [PubMed]

2007 (2)

E. Seli, D. Sakkas, R. Scott, S. C. Kwok, S. M. Rosendahl, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using Raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 88(5), 1350–1357 (2007).
[Crossref] [PubMed]

A. S. Lopes, T. Greve, and H. Callesen, “Quantification of embryo quality by respirometry,” Theriogenology 67(1), 21–31 (2007).
[Crossref] [PubMed]

2004 (1)

D. R. Brison, F. D. Houghton, D. Falconer, S. A. Roberts, J. Hawkhead, P. G. Humpherson, B. A. Lieberman, and H. J. Leese, “Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover,” Hum. Reprod. 19(10), 2319–2324 (2004).
[Crossref] [PubMed]

2002 (2)

M. Shimada, N. Kawano, and T. Terada, “Delay of nuclear maturation and reduction in developmental competence of pig oocytes after mineral oil overlay of in vitro maturation media,” Reproduction 124(4), 557–564 (2002).
[Crossref] [PubMed]

A. Barth and C. Zscherp, “What vibrations tell us about proteins,” Q. Rev. Biophys. 35(4), 369–430 (2002).
[Crossref] [PubMed]

2001 (1)

D. K. Gardner, M. Lane, J. Stevens, and W. B. Schoolcraft, “Noninvasive assessment of human embryo nutrient consumption as a measure of developmental potential,” Fertil. Steril. 76(6), 1175–1180 (2001).
[Crossref] [PubMed]

1987 (1)

D. K. Gardner and H. J. Leese, “Assessment of embryo viability prior to transfer by the noninvasive measurement of glucose uptake,” J. Exp. Zool. 242(1), 103–105 (1987).
[Crossref] [PubMed]

Adiga, S. K.

S. M. Pudakalakatti, S. Uppangala, F. D’Souza, G. Kalthur, P. Kumar, S. K. Adiga, and H. S. Atreya, “NMR studies of preimplantation embryo metabolism in human assisted reproductive techniques: a new biomarker for assessment of embryo implantation potential,” NMR Biomed. 26(1), 20–27 (2013).
[Crossref] [PubMed]

Ahlström, A.

T. Hardarson, A. Ahlström, L. Rogberg, L. Botros, T. Hillensjö, G. Westlander, D. Sakkas, and M. Wikland, “Non-invasive metabolomic profiling of Day 2 and 5 embryo culture medium: a prospective randomized trial,” Hum. Reprod. 27(1), 89–96 (2012).
[Crossref] [PubMed]

Alfano, R. R.

Y. Zhou, C. H. Liu, Y. Sun, Y. Pu, S. Boydston-White, Y. Liu, and R. R. Alfano, “Human brain cancer studied by resonance Raman spectroscopy,” J. Biomed. Opt. 17(11), 116021 (2012).
[Crossref] [PubMed]

Annes, K.

C. A. Soares, K. Annes, T. R. Dreyer, T. Magrini, M. T. Sonoda, H. da Silva Martinho, M. Nichi, M. E. d’Ávila Assumpção, and M. P. Milazzotto, “Photobiological effect of low-level laser irradiation in bovine embryo production system,” J. Biomed. Opt. 19(3), 035006 (2014).
[Crossref] [PubMed]

Ansari, S.

A. Moshaverinia, S. Ansari, Z. Movasaghi, R. W. Billington, J. A. Darr, and I. U. Rehman, “Modification of conventional glass-ionomer cements with N-vinylpyrrolidone containing polyacids, nano-hydroxy and fluoroapatite to improve mechanical properties,” Dent. Mater. 24(10), 1381–1390 (2008).
[Crossref] [PubMed]

Atreya, H. S.

S. M. Pudakalakatti, S. Uppangala, F. D’Souza, G. Kalthur, P. Kumar, S. K. Adiga, and H. S. Atreya, “NMR studies of preimplantation embryo metabolism in human assisted reproductive techniques: a new biomarker for assessment of embryo implantation potential,” NMR Biomed. 26(1), 20–27 (2013).
[Crossref] [PubMed]

Barth, A.

A. Barth and C. Zscherp, “What vibrations tell us about proteins,” Q. Rev. Biophys. 35(4), 369–430 (2002).
[Crossref] [PubMed]

Bergholt, M. S.

M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Yan So, and Z. Huang, “In vivo diagnosis of gastric cancer using Raman endoscopy and ant colony optimization techniques,” Int. J. Cancer 128(11), 2673–2680 (2011).
[Crossref] [PubMed]

Billington, R. W.

A. Moshaverinia, S. Ansari, Z. Movasaghi, R. W. Billington, J. A. Darr, and I. U. Rehman, “Modification of conventional glass-ionomer cements with N-vinylpyrrolidone containing polyacids, nano-hydroxy and fluoroapatite to improve mechanical properties,” Dent. Mater. 24(10), 1381–1390 (2008).
[Crossref] [PubMed]

Borges, E.

S. S. Cortezzi, E. C. Cabral, M. G. Trevisan, C. R. Ferreira, A. S. Setti, D. P. Braga, R. C. Figueira, A. Iaconelli, M. N. Eberlin, and E. Borges., “Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium,” Reproduction 145(5), 453–462 (2013).
[Crossref] [PubMed]

Botros, L.

T. Hardarson, A. Ahlström, L. Rogberg, L. Botros, T. Hillensjö, G. Westlander, D. Sakkas, and M. Wikland, “Non-invasive metabolomic profiling of Day 2 and 5 embryo culture medium: a prospective randomized trial,” Hum. Reprod. 27(1), 89–96 (2012).
[Crossref] [PubMed]

E. Seli, L. Botros, D. Sakkas, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using proton nuclear magnetic resonance correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 90(6), 2183–2189 (2008).
[Crossref] [PubMed]

Botros, L. L.

C. G. Vergouw, L. L. Botros, P. Roos, J. W. Lens, R. Schats, P. G. A. Hompes, D. H. Burns, and C. B. Lambalk, “Metabolomic profiling by near-infrared spectroscopy as a tool to assess embryo viability: a novel, non-invasive method for embryo selection,” Hum. Reprod. 23(7), 1499–1504 (2008).
[Crossref] [PubMed]

Boydston-White, S.

Y. Zhou, C. H. Liu, Y. Sun, Y. Pu, S. Boydston-White, Y. Liu, and R. R. Alfano, “Human brain cancer studied by resonance Raman spectroscopy,” J. Biomed. Opt. 17(11), 116021 (2012).
[Crossref] [PubMed]

Braga, D. P.

S. S. Cortezzi, E. C. Cabral, M. G. Trevisan, C. R. Ferreira, A. S. Setti, D. P. Braga, R. C. Figueira, A. Iaconelli, M. N. Eberlin, and E. Borges., “Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium,” Reproduction 145(5), 453–462 (2013).
[Crossref] [PubMed]

Brennan, L.

M. Wallace, E. Cottell, J. Cullinane, F. M. McAuliffe, M. Wingfield, and L. Brennan, “(1)H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential,” Syst. Biol. Reprod. Med. 60(1), 58–63 (2014).
[Crossref] [PubMed]

Brison, D. R.

D. R. Brison, F. D. Houghton, D. Falconer, S. A. Roberts, J. Hawkhead, P. G. Humpherson, B. A. Lieberman, and H. J. Leese, “Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover,” Hum. Reprod. 19(10), 2319–2324 (2004).
[Crossref] [PubMed]

Burns, D. H.

C. G. Vergouw, L. L. Botros, P. Roos, J. W. Lens, R. Schats, P. G. A. Hompes, D. H. Burns, and C. B. Lambalk, “Metabolomic profiling by near-infrared spectroscopy as a tool to assess embryo viability: a novel, non-invasive method for embryo selection,” Hum. Reprod. 23(7), 1499–1504 (2008).
[Crossref] [PubMed]

E. Seli, L. Botros, D. Sakkas, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using proton nuclear magnetic resonance correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 90(6), 2183–2189 (2008).
[Crossref] [PubMed]

R. Scott, E. Seli, K. Miller, D. Sakkas, K. Scott, and D. H. Burns, “Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study,” Fertil. Steril. 90(1), 77–83 (2008).
[Crossref] [PubMed]

E. Seli, D. Sakkas, R. Scott, S. C. Kwok, S. M. Rosendahl, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using Raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 88(5), 1350–1357 (2007).
[Crossref] [PubMed]

Caamaño, J. N.

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

Cabral, E. C.

S. S. Cortezzi, E. C. Cabral, M. G. Trevisan, C. R. Ferreira, A. S. Setti, D. P. Braga, R. C. Figueira, A. Iaconelli, M. N. Eberlin, and E. Borges., “Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium,” Reproduction 145(5), 453–462 (2013).
[Crossref] [PubMed]

Callesen, H.

A. S. Lopes, T. Greve, and H. Callesen, “Quantification of embryo quality by respirometry,” Theriogenology 67(1), 21–31 (2007).
[Crossref] [PubMed]

Carrocera, S.

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

Chen, M.

L. Xue, P. Sun, D. Ou, P. Chen, M. Chen, and B. Yan, “Diagnosis of pathological minor salivary glands in primary Sjogren’s syndrome by using Raman spectroscopy,” Lasers Med. Sci. 29(2), 723–728 (2014).
[Crossref] [PubMed]

Chen, P.

L. Xue, P. Sun, D. Ou, P. Chen, M. Chen, and B. Yan, “Diagnosis of pathological minor salivary glands in primary Sjogren’s syndrome by using Raman spectroscopy,” Lasers Med. Sci. 29(2), 723–728 (2014).
[Crossref] [PubMed]

Chen, R.

Z. Huang, Y. Sun, J. Wang, S. Du, Y. Li, J. Lin, S. Feng, J. Lei, H. Lin, R. Chen, and H. Zeng, “Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy,” J. Biomed. Opt. 18(12), 127003 (2013).
[Crossref] [PubMed]

Cinta Pinzaru, S.

S. Cinta Pinzaru, A. Falamas, and C. A. Dehelean, “Molecular conformation changes along the malignancy revealed by optical nanosensors,” J. Cell. Mol. Med. 17(2), 277–286 (2013).
[Crossref] [PubMed]

Correia, E.

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

Cortezzi, S. S.

S. S. Cortezzi, E. C. Cabral, M. G. Trevisan, C. R. Ferreira, A. S. Setti, D. P. Braga, R. C. Figueira, A. Iaconelli, M. N. Eberlin, and E. Borges., “Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium,” Reproduction 145(5), 453–462 (2013).
[Crossref] [PubMed]

Cottell, E.

M. Wallace, E. Cottell, J. Cullinane, F. M. McAuliffe, M. Wingfield, and L. Brennan, “(1)H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential,” Syst. Biol. Reprod. Med. 60(1), 58–63 (2014).
[Crossref] [PubMed]

Cuello, C.

C. A. Martinez, A. Nohalez, C. Cuello, J. M. Vazquez, J. Roca, E. A. Martinez, and M. A. Gil, “The use of mineral oil during in vitro maturation, fertilization, and embryo culture does not impair the developmental competence of pig oocytes,” Theriogenology 83(4), 693–702 (2015).
[Crossref] [PubMed]

Cullinane, J.

M. Wallace, E. Cottell, J. Cullinane, F. M. McAuliffe, M. Wingfield, and L. Brennan, “(1)H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential,” Syst. Biol. Reprod. Med. 60(1), 58–63 (2014).
[Crossref] [PubMed]

d’Ávila Assumpção, M. E.

C. A. Soares, K. Annes, T. R. Dreyer, T. Magrini, M. T. Sonoda, H. da Silva Martinho, M. Nichi, M. E. d’Ávila Assumpção, and M. P. Milazzotto, “Photobiological effect of low-level laser irradiation in bovine embryo production system,” J. Biomed. Opt. 19(3), 035006 (2014).
[Crossref] [PubMed]

D’Souza, F.

S. M. Pudakalakatti, S. Uppangala, F. D’Souza, G. Kalthur, P. Kumar, S. K. Adiga, and H. S. Atreya, “NMR studies of preimplantation embryo metabolism in human assisted reproductive techniques: a new biomarker for assessment of embryo implantation potential,” NMR Biomed. 26(1), 20–27 (2013).
[Crossref] [PubMed]

da Silva Martinho, H.

C. A. Soares, K. Annes, T. R. Dreyer, T. Magrini, M. T. Sonoda, H. da Silva Martinho, M. Nichi, M. E. d’Ávila Assumpção, and M. P. Milazzotto, “Photobiological effect of low-level laser irradiation in bovine embryo production system,” J. Biomed. Opt. 19(3), 035006 (2014).
[Crossref] [PubMed]

Darr, J. A.

A. Moshaverinia, S. Ansari, Z. Movasaghi, R. W. Billington, J. A. Darr, and I. U. Rehman, “Modification of conventional glass-ionomer cements with N-vinylpyrrolidone containing polyacids, nano-hydroxy and fluoroapatite to improve mechanical properties,” Dent. Mater. 24(10), 1381–1390 (2008).
[Crossref] [PubMed]

Dehelean, C. A.

S. Cinta Pinzaru, A. Falamas, and C. A. Dehelean, “Molecular conformation changes along the malignancy revealed by optical nanosensors,” J. Cell. Mol. Med. 17(2), 277–286 (2013).
[Crossref] [PubMed]

Diez, C.

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

Dreyer, T. R.

C. A. Soares, K. Annes, T. R. Dreyer, T. Magrini, M. T. Sonoda, H. da Silva Martinho, M. Nichi, M. E. d’Ávila Assumpção, and M. P. Milazzotto, “Photobiological effect of low-level laser irradiation in bovine embryo production system,” J. Biomed. Opt. 19(3), 035006 (2014).
[Crossref] [PubMed]

Du, S.

Z. Huang, Y. Sun, J. Wang, S. Du, Y. Li, J. Lin, S. Feng, J. Lei, H. Lin, R. Chen, and H. Zeng, “Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy,” J. Biomed. Opt. 18(12), 127003 (2013).
[Crossref] [PubMed]

Eberlin, M. N.

S. S. Cortezzi, E. C. Cabral, M. G. Trevisan, C. R. Ferreira, A. S. Setti, D. P. Braga, R. C. Figueira, A. Iaconelli, M. N. Eberlin, and E. Borges., “Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium,” Reproduction 145(5), 453–462 (2013).
[Crossref] [PubMed]

Falamas, A.

S. Cinta Pinzaru, A. Falamas, and C. A. Dehelean, “Molecular conformation changes along the malignancy revealed by optical nanosensors,” J. Cell. Mol. Med. 17(2), 277–286 (2013).
[Crossref] [PubMed]

Falconer, D.

D. R. Brison, F. D. Houghton, D. Falconer, S. A. Roberts, J. Hawkhead, P. G. Humpherson, B. A. Lieberman, and H. J. Leese, “Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover,” Hum. Reprod. 19(10), 2319–2324 (2004).
[Crossref] [PubMed]

Feng, S.

Z. Huang, Y. Sun, J. Wang, S. Du, Y. Li, J. Lin, S. Feng, J. Lei, H. Lin, R. Chen, and H. Zeng, “Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy,” J. Biomed. Opt. 18(12), 127003 (2013).
[Crossref] [PubMed]

Fernandez-gonzales, A.

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

Ferreira, C. R.

S. S. Cortezzi, E. C. Cabral, M. G. Trevisan, C. R. Ferreira, A. S. Setti, D. P. Braga, R. C. Figueira, A. Iaconelli, M. N. Eberlin, and E. Borges., “Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium,” Reproduction 145(5), 453–462 (2013).
[Crossref] [PubMed]

Figueira, R. C.

S. S. Cortezzi, E. C. Cabral, M. G. Trevisan, C. R. Ferreira, A. S. Setti, D. P. Braga, R. C. Figueira, A. Iaconelli, M. N. Eberlin, and E. Borges., “Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium,” Reproduction 145(5), 453–462 (2013).
[Crossref] [PubMed]

Gardner, D. K.

Y. S. Lee, G. A. Thouas, and D. K. Gardner, “Developmental kinetics of cleavage stage mouse embryos are related to their subsequent carbohydrate and amino acid utilization at the blastocyst stage,” Hum. Reprod. 30(3), 543–552 (2015).
[Crossref] [PubMed]

D. K. Gardner, M. Lane, J. Stevens, and W. B. Schoolcraft, “Noninvasive assessment of human embryo nutrient consumption as a measure of developmental potential,” Fertil. Steril. 76(6), 1175–1180 (2001).
[Crossref] [PubMed]

D. K. Gardner and H. J. Leese, “Assessment of embryo viability prior to transfer by the noninvasive measurement of glucose uptake,” J. Exp. Zool. 242(1), 103–105 (1987).
[Crossref] [PubMed]

Gil, M. A.

C. A. Martinez, A. Nohalez, C. Cuello, J. M. Vazquez, J. Roca, E. A. Martinez, and M. A. Gil, “The use of mineral oil during in vitro maturation, fertilization, and embryo culture does not impair the developmental competence of pig oocytes,” Theriogenology 83(4), 693–702 (2015).
[Crossref] [PubMed]

Gomez, E.

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

Greve, T.

A. S. Lopes, T. Greve, and H. Callesen, “Quantification of embryo quality by respirometry,” Theriogenology 67(1), 21–31 (2007).
[Crossref] [PubMed]

Guyader-joly, C.

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

Hardarson, T.

T. Hardarson, A. Ahlström, L. Rogberg, L. Botros, T. Hillensjö, G. Westlander, D. Sakkas, and M. Wikland, “Non-invasive metabolomic profiling of Day 2 and 5 embryo culture medium: a prospective randomized trial,” Hum. Reprod. 27(1), 89–96 (2012).
[Crossref] [PubMed]

Hawkhead, J.

D. R. Brison, F. D. Houghton, D. Falconer, S. A. Roberts, J. Hawkhead, P. G. Humpherson, B. A. Lieberman, and H. J. Leese, “Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover,” Hum. Reprod. 19(10), 2319–2324 (2004).
[Crossref] [PubMed]

Herrero, J.

J. Herrero and M. Meseguer, “Selection of high potential embryos using time-lapse imaging: the era of morphokinetics,” Fertil. Steril. 99(4), 1030–1034 (2013).
[Crossref] [PubMed]

Hillensjö, T.

T. Hardarson, A. Ahlström, L. Rogberg, L. Botros, T. Hillensjö, G. Westlander, D. Sakkas, and M. Wikland, “Non-invasive metabolomic profiling of Day 2 and 5 embryo culture medium: a prospective randomized trial,” Hum. Reprod. 27(1), 89–96 (2012).
[Crossref] [PubMed]

Ho, K. Y.

M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Yan So, and Z. Huang, “In vivo diagnosis of gastric cancer using Raman endoscopy and ant colony optimization techniques,” Int. J. Cancer 128(11), 2673–2680 (2011).
[Crossref] [PubMed]

Hompes, P. G. A.

C. G. Vergouw, L. L. Botros, P. Roos, J. W. Lens, R. Schats, P. G. A. Hompes, D. H. Burns, and C. B. Lambalk, “Metabolomic profiling by near-infrared spectroscopy as a tool to assess embryo viability: a novel, non-invasive method for embryo selection,” Hum. Reprod. 23(7), 1499–1504 (2008).
[Crossref] [PubMed]

Houghton, F. D.

D. R. Brison, F. D. Houghton, D. Falconer, S. A. Roberts, J. Hawkhead, P. G. Humpherson, B. A. Lieberman, and H. J. Leese, “Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover,” Hum. Reprod. 19(10), 2319–2324 (2004).
[Crossref] [PubMed]

Hu, J.

Q. Zhao, T. Yin, J. Peng, Y. Zou, J. Yang, A. Shen, and J. Hu, “Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF),” Int. J. Mol. Sci. 14(4), 6556–6570 (2013).
[Crossref] [PubMed]

Hu, J. M.

A. G. Shen, J. Peng, Q. H. Zhao, L. Su, X. H. Wang, J. M. Hu, and Q. Yang, “Accurate and noninvasive embryos screening during in vitro fertilization (IVF) assisted by Raman analysis of embryos culture medium,” Laser Phys. Lett. 9(4), 322–328 (2012).
[Crossref]

Huang, Z.

Z. Huang, Y. Sun, J. Wang, S. Du, Y. Li, J. Lin, S. Feng, J. Lei, H. Lin, R. Chen, and H. Zeng, “Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy,” J. Biomed. Opt. 18(12), 127003 (2013).
[Crossref] [PubMed]

M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Yan So, and Z. Huang, “In vivo diagnosis of gastric cancer using Raman endoscopy and ant colony optimization techniques,” Int. J. Cancer 128(11), 2673–2680 (2011).
[Crossref] [PubMed]

Humblot, P.

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

Humpherson, P. G.

D. R. Brison, F. D. Houghton, D. Falconer, S. A. Roberts, J. Hawkhead, P. G. Humpherson, B. A. Lieberman, and H. J. Leese, “Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover,” Hum. Reprod. 19(10), 2319–2324 (2004).
[Crossref] [PubMed]

Iaconelli, A.

S. S. Cortezzi, E. C. Cabral, M. G. Trevisan, C. R. Ferreira, A. S. Setti, D. P. Braga, R. C. Figueira, A. Iaconelli, M. N. Eberlin, and E. Borges., “Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium,” Reproduction 145(5), 453–462 (2013).
[Crossref] [PubMed]

Jonáš, A.

O. Samek, A. Jonáš, Z. Pilát, P. Zemánek, L. Nedbal, J. Tříska, P. Kotas, and M. Trtílek, “Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo,” Sensors (Basel) 10(9), 8635–8651 (2010).
[Crossref] [PubMed]

Kalthur, G.

S. M. Pudakalakatti, S. Uppangala, F. D’Souza, G. Kalthur, P. Kumar, S. K. Adiga, and H. S. Atreya, “NMR studies of preimplantation embryo metabolism in human assisted reproductive techniques: a new biomarker for assessment of embryo implantation potential,” NMR Biomed. 26(1), 20–27 (2013).
[Crossref] [PubMed]

Kawano, N.

M. Shimada, N. Kawano, and T. Terada, “Delay of nuclear maturation and reduction in developmental competence of pig oocytes after mineral oil overlay of in vitro maturation media,” Reproduction 124(4), 557–564 (2002).
[Crossref] [PubMed]

Kotas, P.

O. Samek, A. Jonáš, Z. Pilát, P. Zemánek, L. Nedbal, J. Tříska, P. Kotas, and M. Trtílek, “Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo,” Sensors (Basel) 10(9), 8635–8651 (2010).
[Crossref] [PubMed]

Kumar, P.

S. M. Pudakalakatti, S. Uppangala, F. D’Souza, G. Kalthur, P. Kumar, S. K. Adiga, and H. S. Atreya, “NMR studies of preimplantation embryo metabolism in human assisted reproductive techniques: a new biomarker for assessment of embryo implantation potential,” NMR Biomed. 26(1), 20–27 (2013).
[Crossref] [PubMed]

Kwok, S. C.

E. Seli, D. Sakkas, R. Scott, S. C. Kwok, S. M. Rosendahl, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using Raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 88(5), 1350–1357 (2007).
[Crossref] [PubMed]

Lambalk, C. B.

C. G. Vergouw, L. L. Botros, P. Roos, J. W. Lens, R. Schats, P. G. A. Hompes, D. H. Burns, and C. B. Lambalk, “Metabolomic profiling by near-infrared spectroscopy as a tool to assess embryo viability: a novel, non-invasive method for embryo selection,” Hum. Reprod. 23(7), 1499–1504 (2008).
[Crossref] [PubMed]

Lane, M.

D. K. Gardner, M. Lane, J. Stevens, and W. B. Schoolcraft, “Noninvasive assessment of human embryo nutrient consumption as a measure of developmental potential,” Fertil. Steril. 76(6), 1175–1180 (2001).
[Crossref] [PubMed]

Lee, Y. S.

Y. S. Lee, G. A. Thouas, and D. K. Gardner, “Developmental kinetics of cleavage stage mouse embryos are related to their subsequent carbohydrate and amino acid utilization at the blastocyst stage,” Hum. Reprod. 30(3), 543–552 (2015).
[Crossref] [PubMed]

Leese, H. J.

D. R. Brison, F. D. Houghton, D. Falconer, S. A. Roberts, J. Hawkhead, P. G. Humpherson, B. A. Lieberman, and H. J. Leese, “Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover,” Hum. Reprod. 19(10), 2319–2324 (2004).
[Crossref] [PubMed]

D. K. Gardner and H. J. Leese, “Assessment of embryo viability prior to transfer by the noninvasive measurement of glucose uptake,” J. Exp. Zool. 242(1), 103–105 (1987).
[Crossref] [PubMed]

Lei, J.

Z. Huang, Y. Sun, J. Wang, S. Du, Y. Li, J. Lin, S. Feng, J. Lei, H. Lin, R. Chen, and H. Zeng, “Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy,” J. Biomed. Opt. 18(12), 127003 (2013).
[Crossref] [PubMed]

Lens, J. W.

C. G. Vergouw, L. L. Botros, P. Roos, J. W. Lens, R. Schats, P. G. A. Hompes, D. H. Burns, and C. B. Lambalk, “Metabolomic profiling by near-infrared spectroscopy as a tool to assess embryo viability: a novel, non-invasive method for embryo selection,” Hum. Reprod. 23(7), 1499–1504 (2008).
[Crossref] [PubMed]

Li, Y.

Z. Huang, Y. Sun, J. Wang, S. Du, Y. Li, J. Lin, S. Feng, J. Lei, H. Lin, R. Chen, and H. Zeng, “Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy,” J. Biomed. Opt. 18(12), 127003 (2013).
[Crossref] [PubMed]

Lieberman, B. A.

D. R. Brison, F. D. Houghton, D. Falconer, S. A. Roberts, J. Hawkhead, P. G. Humpherson, B. A. Lieberman, and H. J. Leese, “Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover,” Hum. Reprod. 19(10), 2319–2324 (2004).
[Crossref] [PubMed]

Lin, H.

Z. Huang, Y. Sun, J. Wang, S. Du, Y. Li, J. Lin, S. Feng, J. Lei, H. Lin, R. Chen, and H. Zeng, “Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy,” J. Biomed. Opt. 18(12), 127003 (2013).
[Crossref] [PubMed]

Lin, J.

Z. Huang, Y. Sun, J. Wang, S. Du, Y. Li, J. Lin, S. Feng, J. Lei, H. Lin, R. Chen, and H. Zeng, “Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy,” J. Biomed. Opt. 18(12), 127003 (2013).
[Crossref] [PubMed]

Lin, K.

M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Yan So, and Z. Huang, “In vivo diagnosis of gastric cancer using Raman endoscopy and ant colony optimization techniques,” Int. J. Cancer 128(11), 2673–2680 (2011).
[Crossref] [PubMed]

Liu, C. H.

Y. Zhou, C. H. Liu, Y. Sun, Y. Pu, S. Boydston-White, Y. Liu, and R. R. Alfano, “Human brain cancer studied by resonance Raman spectroscopy,” J. Biomed. Opt. 17(11), 116021 (2012).
[Crossref] [PubMed]

Liu, Y.

Y. Zhou, C. H. Liu, Y. Sun, Y. Pu, S. Boydston-White, Y. Liu, and R. R. Alfano, “Human brain cancer studied by resonance Raman spectroscopy,” J. Biomed. Opt. 17(11), 116021 (2012).
[Crossref] [PubMed]

Lopes, A. S.

A. S. Lopes, T. Greve, and H. Callesen, “Quantification of embryo quality by respirometry,” Theriogenology 67(1), 21–31 (2007).
[Crossref] [PubMed]

Magrini, T.

C. A. Soares, K. Annes, T. R. Dreyer, T. Magrini, M. T. Sonoda, H. da Silva Martinho, M. Nichi, M. E. d’Ávila Assumpção, and M. P. Milazzotto, “Photobiological effect of low-level laser irradiation in bovine embryo production system,” J. Biomed. Opt. 19(3), 035006 (2014).
[Crossref] [PubMed]

Marquant Le Guienne, B.

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

Martin, D.

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

Martinez, C. A.

C. A. Martinez, A. Nohalez, C. Cuello, J. M. Vazquez, J. Roca, E. A. Martinez, and M. A. Gil, “The use of mineral oil during in vitro maturation, fertilization, and embryo culture does not impair the developmental competence of pig oocytes,” Theriogenology 83(4), 693–702 (2015).
[Crossref] [PubMed]

Martinez, E. A.

C. A. Martinez, A. Nohalez, C. Cuello, J. M. Vazquez, J. Roca, E. A. Martinez, and M. A. Gil, “The use of mineral oil during in vitro maturation, fertilization, and embryo culture does not impair the developmental competence of pig oocytes,” Theriogenology 83(4), 693–702 (2015).
[Crossref] [PubMed]

Martinez-bello, D.

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

McAuliffe, F. M.

M. Wallace, E. Cottell, J. Cullinane, F. M. McAuliffe, M. Wingfield, and L. Brennan, “(1)H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential,” Syst. Biol. Reprod. Med. 60(1), 58–63 (2014).
[Crossref] [PubMed]

Meseguer, M.

J. Herrero and M. Meseguer, “Selection of high potential embryos using time-lapse imaging: the era of morphokinetics,” Fertil. Steril. 99(4), 1030–1034 (2013).
[Crossref] [PubMed]

Milazzotto, M. P.

C. A. Soares, K. Annes, T. R. Dreyer, T. Magrini, M. T. Sonoda, H. da Silva Martinho, M. Nichi, M. E. d’Ávila Assumpção, and M. P. Milazzotto, “Photobiological effect of low-level laser irradiation in bovine embryo production system,” J. Biomed. Opt. 19(3), 035006 (2014).
[Crossref] [PubMed]

Miller, K.

R. Scott, E. Seli, K. Miller, D. Sakkas, K. Scott, and D. H. Burns, “Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study,” Fertil. Steril. 90(1), 77–83 (2008).
[Crossref] [PubMed]

Moshaverinia, A.

A. Moshaverinia, S. Ansari, Z. Movasaghi, R. W. Billington, J. A. Darr, and I. U. Rehman, “Modification of conventional glass-ionomer cements with N-vinylpyrrolidone containing polyacids, nano-hydroxy and fluoroapatite to improve mechanical properties,” Dent. Mater. 24(10), 1381–1390 (2008).
[Crossref] [PubMed]

Movasaghi, Z.

A. Moshaverinia, S. Ansari, Z. Movasaghi, R. W. Billington, J. A. Darr, and I. U. Rehman, “Modification of conventional glass-ionomer cements with N-vinylpyrrolidone containing polyacids, nano-hydroxy and fluoroapatite to improve mechanical properties,” Dent. Mater. 24(10), 1381–1390 (2008).
[Crossref] [PubMed]

Muñoz, M.

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

Nedbal, L.

O. Samek, A. Jonáš, Z. Pilát, P. Zemánek, L. Nedbal, J. Tříska, P. Kotas, and M. Trtílek, “Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo,” Sensors (Basel) 10(9), 8635–8651 (2010).
[Crossref] [PubMed]

Nichi, M.

C. A. Soares, K. Annes, T. R. Dreyer, T. Magrini, M. T. Sonoda, H. da Silva Martinho, M. Nichi, M. E. d’Ávila Assumpção, and M. P. Milazzotto, “Photobiological effect of low-level laser irradiation in bovine embryo production system,” J. Biomed. Opt. 19(3), 035006 (2014).
[Crossref] [PubMed]

Nohalez, A.

C. A. Martinez, A. Nohalez, C. Cuello, J. M. Vazquez, J. Roca, E. A. Martinez, and M. A. Gil, “The use of mineral oil during in vitro maturation, fertilization, and embryo culture does not impair the developmental competence of pig oocytes,” Theriogenology 83(4), 693–702 (2015).
[Crossref] [PubMed]

Ou, D.

L. Xue, P. Sun, D. Ou, P. Chen, M. Chen, and B. Yan, “Diagnosis of pathological minor salivary glands in primary Sjogren’s syndrome by using Raman spectroscopy,” Lasers Med. Sci. 29(2), 723–728 (2014).
[Crossref] [PubMed]

Peng, J.

Q. Zhao, T. Yin, J. Peng, Y. Zou, J. Yang, A. Shen, and J. Hu, “Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF),” Int. J. Mol. Sci. 14(4), 6556–6570 (2013).
[Crossref] [PubMed]

A. G. Shen, J. Peng, Q. H. Zhao, L. Su, X. H. Wang, J. M. Hu, and Q. Yang, “Accurate and noninvasive embryos screening during in vitro fertilization (IVF) assisted by Raman analysis of embryos culture medium,” Laser Phys. Lett. 9(4), 322–328 (2012).
[Crossref]

Pilát, Z.

O. Samek, A. Jonáš, Z. Pilát, P. Zemánek, L. Nedbal, J. Tříska, P. Kotas, and M. Trtílek, “Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo,” Sensors (Basel) 10(9), 8635–8651 (2010).
[Crossref] [PubMed]

Ponsart, C.

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

Pu, Y.

Y. Zhou, C. H. Liu, Y. Sun, Y. Pu, S. Boydston-White, Y. Liu, and R. R. Alfano, “Human brain cancer studied by resonance Raman spectroscopy,” J. Biomed. Opt. 17(11), 116021 (2012).
[Crossref] [PubMed]

Pudakalakatti, S. M.

S. M. Pudakalakatti, S. Uppangala, F. D’Souza, G. Kalthur, P. Kumar, S. K. Adiga, and H. S. Atreya, “NMR studies of preimplantation embryo metabolism in human assisted reproductive techniques: a new biomarker for assessment of embryo implantation potential,” NMR Biomed. 26(1), 20–27 (2013).
[Crossref] [PubMed]

Rehman, I. U.

A. Moshaverinia, S. Ansari, Z. Movasaghi, R. W. Billington, J. A. Darr, and I. U. Rehman, “Modification of conventional glass-ionomer cements with N-vinylpyrrolidone containing polyacids, nano-hydroxy and fluoroapatite to improve mechanical properties,” Dent. Mater. 24(10), 1381–1390 (2008).
[Crossref] [PubMed]

Roberts, S. A.

D. R. Brison, F. D. Houghton, D. Falconer, S. A. Roberts, J. Hawkhead, P. G. Humpherson, B. A. Lieberman, and H. J. Leese, “Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover,” Hum. Reprod. 19(10), 2319–2324 (2004).
[Crossref] [PubMed]

Roca, J.

C. A. Martinez, A. Nohalez, C. Cuello, J. M. Vazquez, J. Roca, E. A. Martinez, and M. A. Gil, “The use of mineral oil during in vitro maturation, fertilization, and embryo culture does not impair the developmental competence of pig oocytes,” Theriogenology 83(4), 693–702 (2015).
[Crossref] [PubMed]

Rogberg, L.

T. Hardarson, A. Ahlström, L. Rogberg, L. Botros, T. Hillensjö, G. Westlander, D. Sakkas, and M. Wikland, “Non-invasive metabolomic profiling of Day 2 and 5 embryo culture medium: a prospective randomized trial,” Hum. Reprod. 27(1), 89–96 (2012).
[Crossref] [PubMed]

Roos, P.

C. G. Vergouw, L. L. Botros, P. Roos, J. W. Lens, R. Schats, P. G. A. Hompes, D. H. Burns, and C. B. Lambalk, “Metabolomic profiling by near-infrared spectroscopy as a tool to assess embryo viability: a novel, non-invasive method for embryo selection,” Hum. Reprod. 23(7), 1499–1504 (2008).
[Crossref] [PubMed]

Rosendahl, S. M.

E. Seli, D. Sakkas, R. Scott, S. C. Kwok, S. M. Rosendahl, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using Raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 88(5), 1350–1357 (2007).
[Crossref] [PubMed]

Sakkas, D.

T. Hardarson, A. Ahlström, L. Rogberg, L. Botros, T. Hillensjö, G. Westlander, D. Sakkas, and M. Wikland, “Non-invasive metabolomic profiling of Day 2 and 5 embryo culture medium: a prospective randomized trial,” Hum. Reprod. 27(1), 89–96 (2012).
[Crossref] [PubMed]

R. Scott, E. Seli, K. Miller, D. Sakkas, K. Scott, and D. H. Burns, “Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study,” Fertil. Steril. 90(1), 77–83 (2008).
[Crossref] [PubMed]

E. Seli, L. Botros, D. Sakkas, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using proton nuclear magnetic resonance correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 90(6), 2183–2189 (2008).
[Crossref] [PubMed]

E. Seli, D. Sakkas, R. Scott, S. C. Kwok, S. M. Rosendahl, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using Raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 88(5), 1350–1357 (2007).
[Crossref] [PubMed]

Samek, O.

O. Samek, A. Jonáš, Z. Pilát, P. Zemánek, L. Nedbal, J. Tříska, P. Kotas, and M. Trtílek, “Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo,” Sensors (Basel) 10(9), 8635–8651 (2010).
[Crossref] [PubMed]

Schats, R.

C. G. Vergouw, L. L. Botros, P. Roos, J. W. Lens, R. Schats, P. G. A. Hompes, D. H. Burns, and C. B. Lambalk, “Metabolomic profiling by near-infrared spectroscopy as a tool to assess embryo viability: a novel, non-invasive method for embryo selection,” Hum. Reprod. 23(7), 1499–1504 (2008).
[Crossref] [PubMed]

Schoolcraft, W. B.

D. K. Gardner, M. Lane, J. Stevens, and W. B. Schoolcraft, “Noninvasive assessment of human embryo nutrient consumption as a measure of developmental potential,” Fertil. Steril. 76(6), 1175–1180 (2001).
[Crossref] [PubMed]

Scott, K.

R. Scott, E. Seli, K. Miller, D. Sakkas, K. Scott, and D. H. Burns, “Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study,” Fertil. Steril. 90(1), 77–83 (2008).
[Crossref] [PubMed]

Scott, R.

R. Scott, E. Seli, K. Miller, D. Sakkas, K. Scott, and D. H. Burns, “Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study,” Fertil. Steril. 90(1), 77–83 (2008).
[Crossref] [PubMed]

E. Seli, D. Sakkas, R. Scott, S. C. Kwok, S. M. Rosendahl, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using Raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 88(5), 1350–1357 (2007).
[Crossref] [PubMed]

Seli, E.

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

E. Seli, L. Botros, D. Sakkas, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using proton nuclear magnetic resonance correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 90(6), 2183–2189 (2008).
[Crossref] [PubMed]

R. Scott, E. Seli, K. Miller, D. Sakkas, K. Scott, and D. H. Burns, “Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study,” Fertil. Steril. 90(1), 77–83 (2008).
[Crossref] [PubMed]

E. Seli, D. Sakkas, R. Scott, S. C. Kwok, S. M. Rosendahl, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using Raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 88(5), 1350–1357 (2007).
[Crossref] [PubMed]

Setti, A. S.

S. S. Cortezzi, E. C. Cabral, M. G. Trevisan, C. R. Ferreira, A. S. Setti, D. P. Braga, R. C. Figueira, A. Iaconelli, M. N. Eberlin, and E. Borges., “Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium,” Reproduction 145(5), 453–462 (2013).
[Crossref] [PubMed]

Shen, A.

Q. Zhao, T. Yin, J. Peng, Y. Zou, J. Yang, A. Shen, and J. Hu, “Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF),” Int. J. Mol. Sci. 14(4), 6556–6570 (2013).
[Crossref] [PubMed]

Shen, A. G.

A. G. Shen, J. Peng, Q. H. Zhao, L. Su, X. H. Wang, J. M. Hu, and Q. Yang, “Accurate and noninvasive embryos screening during in vitro fertilization (IVF) assisted by Raman analysis of embryos culture medium,” Laser Phys. Lett. 9(4), 322–328 (2012).
[Crossref]

Shimada, M.

M. Shimada, N. Kawano, and T. Terada, “Delay of nuclear maturation and reduction in developmental competence of pig oocytes after mineral oil overlay of in vitro maturation media,” Reproduction 124(4), 557–564 (2002).
[Crossref] [PubMed]

Soares, C. A.

C. A. Soares, K. Annes, T. R. Dreyer, T. Magrini, M. T. Sonoda, H. da Silva Martinho, M. Nichi, M. E. d’Ávila Assumpção, and M. P. Milazzotto, “Photobiological effect of low-level laser irradiation in bovine embryo production system,” J. Biomed. Opt. 19(3), 035006 (2014).
[Crossref] [PubMed]

Sonoda, M. T.

C. A. Soares, K. Annes, T. R. Dreyer, T. Magrini, M. T. Sonoda, H. da Silva Martinho, M. Nichi, M. E. d’Ávila Assumpção, and M. P. Milazzotto, “Photobiological effect of low-level laser irradiation in bovine embryo production system,” J. Biomed. Opt. 19(3), 035006 (2014).
[Crossref] [PubMed]

Stevens, J.

D. K. Gardner, M. Lane, J. Stevens, and W. B. Schoolcraft, “Noninvasive assessment of human embryo nutrient consumption as a measure of developmental potential,” Fertil. Steril. 76(6), 1175–1180 (2001).
[Crossref] [PubMed]

Su, L.

A. G. Shen, J. Peng, Q. H. Zhao, L. Su, X. H. Wang, J. M. Hu, and Q. Yang, “Accurate and noninvasive embryos screening during in vitro fertilization (IVF) assisted by Raman analysis of embryos culture medium,” Laser Phys. Lett. 9(4), 322–328 (2012).
[Crossref]

Sun, P.

L. Xue, P. Sun, D. Ou, P. Chen, M. Chen, and B. Yan, “Diagnosis of pathological minor salivary glands in primary Sjogren’s syndrome by using Raman spectroscopy,” Lasers Med. Sci. 29(2), 723–728 (2014).
[Crossref] [PubMed]

Sun, Y.

Z. Huang, Y. Sun, J. Wang, S. Du, Y. Li, J. Lin, S. Feng, J. Lei, H. Lin, R. Chen, and H. Zeng, “Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy,” J. Biomed. Opt. 18(12), 127003 (2013).
[Crossref] [PubMed]

Y. Zhou, C. H. Liu, Y. Sun, Y. Pu, S. Boydston-White, Y. Liu, and R. R. Alfano, “Human brain cancer studied by resonance Raman spectroscopy,” J. Biomed. Opt. 17(11), 116021 (2012).
[Crossref] [PubMed]

Teh, M.

M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Yan So, and Z. Huang, “In vivo diagnosis of gastric cancer using Raman endoscopy and ant colony optimization techniques,” Int. J. Cancer 128(11), 2673–2680 (2011).
[Crossref] [PubMed]

Terada, T.

M. Shimada, N. Kawano, and T. Terada, “Delay of nuclear maturation and reduction in developmental competence of pig oocytes after mineral oil overlay of in vitro maturation media,” Reproduction 124(4), 557–564 (2002).
[Crossref] [PubMed]

Thouas, G. A.

Y. S. Lee, G. A. Thouas, and D. K. Gardner, “Developmental kinetics of cleavage stage mouse embryos are related to their subsequent carbohydrate and amino acid utilization at the blastocyst stage,” Hum. Reprod. 30(3), 543–552 (2015).
[Crossref] [PubMed]

Trevisan, M. G.

S. S. Cortezzi, E. C. Cabral, M. G. Trevisan, C. R. Ferreira, A. S. Setti, D. P. Braga, R. C. Figueira, A. Iaconelli, M. N. Eberlin, and E. Borges., “Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium,” Reproduction 145(5), 453–462 (2013).
[Crossref] [PubMed]

Trigal, B.

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

Tríska, J.

O. Samek, A. Jonáš, Z. Pilát, P. Zemánek, L. Nedbal, J. Tříska, P. Kotas, and M. Trtílek, “Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo,” Sensors (Basel) 10(9), 8635–8651 (2010).
[Crossref] [PubMed]

Trtílek, M.

O. Samek, A. Jonáš, Z. Pilát, P. Zemánek, L. Nedbal, J. Tříska, P. Kotas, and M. Trtílek, “Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo,” Sensors (Basel) 10(9), 8635–8651 (2010).
[Crossref] [PubMed]

Uppangala, S.

S. M. Pudakalakatti, S. Uppangala, F. D’Souza, G. Kalthur, P. Kumar, S. K. Adiga, and H. S. Atreya, “NMR studies of preimplantation embryo metabolism in human assisted reproductive techniques: a new biomarker for assessment of embryo implantation potential,” NMR Biomed. 26(1), 20–27 (2013).
[Crossref] [PubMed]

Uuyar, A.

M. Muñoz, A. Uuyar, E. Correia, C. Diez, A. Fernandez-gonzales, J. N. Caamaño, D. Martinez-bello, B. Trigal, P. Humblot, C. Ponsart, C. Guyader-joly, S. Carrocera, D. Martin, B. Marquant Le Guienne, E. Seli, and E. Gomez, “Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy,” J. Dairy Sci. 97(9), 1–8 (2014).

Vazquez, J. M.

C. A. Martinez, A. Nohalez, C. Cuello, J. M. Vazquez, J. Roca, E. A. Martinez, and M. A. Gil, “The use of mineral oil during in vitro maturation, fertilization, and embryo culture does not impair the developmental competence of pig oocytes,” Theriogenology 83(4), 693–702 (2015).
[Crossref] [PubMed]

Vergouw, C. G.

C. G. Vergouw, L. L. Botros, P. Roos, J. W. Lens, R. Schats, P. G. A. Hompes, D. H. Burns, and C. B. Lambalk, “Metabolomic profiling by near-infrared spectroscopy as a tool to assess embryo viability: a novel, non-invasive method for embryo selection,” Hum. Reprod. 23(7), 1499–1504 (2008).
[Crossref] [PubMed]

Wallace, M.

M. Wallace, E. Cottell, J. Cullinane, F. M. McAuliffe, M. Wingfield, and L. Brennan, “(1)H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential,” Syst. Biol. Reprod. Med. 60(1), 58–63 (2014).
[Crossref] [PubMed]

Wang, J.

Z. Huang, Y. Sun, J. Wang, S. Du, Y. Li, J. Lin, S. Feng, J. Lei, H. Lin, R. Chen, and H. Zeng, “Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy,” J. Biomed. Opt. 18(12), 127003 (2013).
[Crossref] [PubMed]

Wang, X. H.

A. G. Shen, J. Peng, Q. H. Zhao, L. Su, X. H. Wang, J. M. Hu, and Q. Yang, “Accurate and noninvasive embryos screening during in vitro fertilization (IVF) assisted by Raman analysis of embryos culture medium,” Laser Phys. Lett. 9(4), 322–328 (2012).
[Crossref]

Westlander, G.

T. Hardarson, A. Ahlström, L. Rogberg, L. Botros, T. Hillensjö, G. Westlander, D. Sakkas, and M. Wikland, “Non-invasive metabolomic profiling of Day 2 and 5 embryo culture medium: a prospective randomized trial,” Hum. Reprod. 27(1), 89–96 (2012).
[Crossref] [PubMed]

Wikland, M.

T. Hardarson, A. Ahlström, L. Rogberg, L. Botros, T. Hillensjö, G. Westlander, D. Sakkas, and M. Wikland, “Non-invasive metabolomic profiling of Day 2 and 5 embryo culture medium: a prospective randomized trial,” Hum. Reprod. 27(1), 89–96 (2012).
[Crossref] [PubMed]

Wingfield, M.

M. Wallace, E. Cottell, J. Cullinane, F. M. McAuliffe, M. Wingfield, and L. Brennan, “(1)H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential,” Syst. Biol. Reprod. Med. 60(1), 58–63 (2014).
[Crossref] [PubMed]

Wojdyr, M.

M. Wojdyr, “Fityk: a general-purpose peak fitting program,” J. Appl. Cryst. 43(5), 1126–1128 (2010).
[Crossref]

Xue, L.

L. Xue, P. Sun, D. Ou, P. Chen, M. Chen, and B. Yan, “Diagnosis of pathological minor salivary glands in primary Sjogren’s syndrome by using Raman spectroscopy,” Lasers Med. Sci. 29(2), 723–728 (2014).
[Crossref] [PubMed]

Yan, B.

L. Xue, P. Sun, D. Ou, P. Chen, M. Chen, and B. Yan, “Diagnosis of pathological minor salivary glands in primary Sjogren’s syndrome by using Raman spectroscopy,” Lasers Med. Sci. 29(2), 723–728 (2014).
[Crossref] [PubMed]

Yan So, J. B.

M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Yan So, and Z. Huang, “In vivo diagnosis of gastric cancer using Raman endoscopy and ant colony optimization techniques,” Int. J. Cancer 128(11), 2673–2680 (2011).
[Crossref] [PubMed]

Yang, J.

Q. Zhao, T. Yin, J. Peng, Y. Zou, J. Yang, A. Shen, and J. Hu, “Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF),” Int. J. Mol. Sci. 14(4), 6556–6570 (2013).
[Crossref] [PubMed]

Yang, Q.

A. G. Shen, J. Peng, Q. H. Zhao, L. Su, X. H. Wang, J. M. Hu, and Q. Yang, “Accurate and noninvasive embryos screening during in vitro fertilization (IVF) assisted by Raman analysis of embryos culture medium,” Laser Phys. Lett. 9(4), 322–328 (2012).
[Crossref]

Yeoh, K. G.

M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Yan So, and Z. Huang, “In vivo diagnosis of gastric cancer using Raman endoscopy and ant colony optimization techniques,” Int. J. Cancer 128(11), 2673–2680 (2011).
[Crossref] [PubMed]

Yin, T.

Q. Zhao, T. Yin, J. Peng, Y. Zou, J. Yang, A. Shen, and J. Hu, “Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF),” Int. J. Mol. Sci. 14(4), 6556–6570 (2013).
[Crossref] [PubMed]

Zemánek, P.

O. Samek, A. Jonáš, Z. Pilát, P. Zemánek, L. Nedbal, J. Tříska, P. Kotas, and M. Trtílek, “Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo,” Sensors (Basel) 10(9), 8635–8651 (2010).
[Crossref] [PubMed]

Zeng, H.

Z. Huang, Y. Sun, J. Wang, S. Du, Y. Li, J. Lin, S. Feng, J. Lei, H. Lin, R. Chen, and H. Zeng, “Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy,” J. Biomed. Opt. 18(12), 127003 (2013).
[Crossref] [PubMed]

Zhao, Q.

Q. Zhao, T. Yin, J. Peng, Y. Zou, J. Yang, A. Shen, and J. Hu, “Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF),” Int. J. Mol. Sci. 14(4), 6556–6570 (2013).
[Crossref] [PubMed]

Zhao, Q. H.

A. G. Shen, J. Peng, Q. H. Zhao, L. Su, X. H. Wang, J. M. Hu, and Q. Yang, “Accurate and noninvasive embryos screening during in vitro fertilization (IVF) assisted by Raman analysis of embryos culture medium,” Laser Phys. Lett. 9(4), 322–328 (2012).
[Crossref]

Zheng, W.

M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Yan So, and Z. Huang, “In vivo diagnosis of gastric cancer using Raman endoscopy and ant colony optimization techniques,” Int. J. Cancer 128(11), 2673–2680 (2011).
[Crossref] [PubMed]

Zhou, Y.

Y. Zhou, C. H. Liu, Y. Sun, Y. Pu, S. Boydston-White, Y. Liu, and R. R. Alfano, “Human brain cancer studied by resonance Raman spectroscopy,” J. Biomed. Opt. 17(11), 116021 (2012).
[Crossref] [PubMed]

Zou, Y.

Q. Zhao, T. Yin, J. Peng, Y. Zou, J. Yang, A. Shen, and J. Hu, “Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF),” Int. J. Mol. Sci. 14(4), 6556–6570 (2013).
[Crossref] [PubMed]

Zscherp, C.

A. Barth and C. Zscherp, “What vibrations tell us about proteins,” Q. Rev. Biophys. 35(4), 369–430 (2002).
[Crossref] [PubMed]

Dent. Mater. (1)

A. Moshaverinia, S. Ansari, Z. Movasaghi, R. W. Billington, J. A. Darr, and I. U. Rehman, “Modification of conventional glass-ionomer cements with N-vinylpyrrolidone containing polyacids, nano-hydroxy and fluoroapatite to improve mechanical properties,” Dent. Mater. 24(10), 1381–1390 (2008).
[Crossref] [PubMed]

Fertil. Steril. (5)

E. Seli, L. Botros, D. Sakkas, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using proton nuclear magnetic resonance correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 90(6), 2183–2189 (2008).
[Crossref] [PubMed]

J. Herrero and M. Meseguer, “Selection of high potential embryos using time-lapse imaging: the era of morphokinetics,” Fertil. Steril. 99(4), 1030–1034 (2013).
[Crossref] [PubMed]

D. K. Gardner, M. Lane, J. Stevens, and W. B. Schoolcraft, “Noninvasive assessment of human embryo nutrient consumption as a measure of developmental potential,” Fertil. Steril. 76(6), 1175–1180 (2001).
[Crossref] [PubMed]

E. Seli, D. Sakkas, R. Scott, S. C. Kwok, S. M. Rosendahl, and D. H. Burns, “Noninvasive metabolomic profiling of embryo culture media using Raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization,” Fertil. Steril. 88(5), 1350–1357 (2007).
[Crossref] [PubMed]

R. Scott, E. Seli, K. Miller, D. Sakkas, K. Scott, and D. H. Burns, “Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study,” Fertil. Steril. 90(1), 77–83 (2008).
[Crossref] [PubMed]

Hum. Reprod. (4)

T. Hardarson, A. Ahlström, L. Rogberg, L. Botros, T. Hillensjö, G. Westlander, D. Sakkas, and M. Wikland, “Non-invasive metabolomic profiling of Day 2 and 5 embryo culture medium: a prospective randomized trial,” Hum. Reprod. 27(1), 89–96 (2012).
[Crossref] [PubMed]

Y. S. Lee, G. A. Thouas, and D. K. Gardner, “Developmental kinetics of cleavage stage mouse embryos are related to their subsequent carbohydrate and amino acid utilization at the blastocyst stage,” Hum. Reprod. 30(3), 543–552 (2015).
[Crossref] [PubMed]

D. R. Brison, F. D. Houghton, D. Falconer, S. A. Roberts, J. Hawkhead, P. G. Humpherson, B. A. Lieberman, and H. J. Leese, “Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover,” Hum. Reprod. 19(10), 2319–2324 (2004).
[Crossref] [PubMed]

C. G. Vergouw, L. L. Botros, P. Roos, J. W. Lens, R. Schats, P. G. A. Hompes, D. H. Burns, and C. B. Lambalk, “Metabolomic profiling by near-infrared spectroscopy as a tool to assess embryo viability: a novel, non-invasive method for embryo selection,” Hum. Reprod. 23(7), 1499–1504 (2008).
[Crossref] [PubMed]

Int. J. Cancer (1)

M. S. Bergholt, W. Zheng, K. Lin, K. Y. Ho, M. Teh, K. G. Yeoh, J. B. Yan So, and Z. Huang, “In vivo diagnosis of gastric cancer using Raman endoscopy and ant colony optimization techniques,” Int. J. Cancer 128(11), 2673–2680 (2011).
[Crossref] [PubMed]

Int. J. Mol. Sci. (1)

Q. Zhao, T. Yin, J. Peng, Y. Zou, J. Yang, A. Shen, and J. Hu, “Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF),” Int. J. Mol. Sci. 14(4), 6556–6570 (2013).
[Crossref] [PubMed]

J. Appl. Cryst. (1)

M. Wojdyr, “Fityk: a general-purpose peak fitting program,” J. Appl. Cryst. 43(5), 1126–1128 (2010).
[Crossref]

J. Biomed. Opt. (3)

Y. Zhou, C. H. Liu, Y. Sun, Y. Pu, S. Boydston-White, Y. Liu, and R. R. Alfano, “Human brain cancer studied by resonance Raman spectroscopy,” J. Biomed. Opt. 17(11), 116021 (2012).
[Crossref] [PubMed]

C. A. Soares, K. Annes, T. R. Dreyer, T. Magrini, M. T. Sonoda, H. da Silva Martinho, M. Nichi, M. E. d’Ávila Assumpção, and M. P. Milazzotto, “Photobiological effect of low-level laser irradiation in bovine embryo production system,” J. Biomed. Opt. 19(3), 035006 (2014).
[Crossref] [PubMed]

Z. Huang, Y. Sun, J. Wang, S. Du, Y. Li, J. Lin, S. Feng, J. Lei, H. Lin, R. Chen, and H. Zeng, “Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy,” J. Biomed. Opt. 18(12), 127003 (2013).
[Crossref] [PubMed]

J. Cell. Mol. Med. (1)

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Figures (6)

Fig. 1
Fig. 1 Schematic diagram of the proposed experimental model. A) culture medium droplets covered with mineral oil on Petri dish in the micro-Raman system; B) incidence of the laser beam on the sample with the spectra acquisition.
Fig. 2
Fig. 2 Oil-culture interface identification in the droplet. The two highlighted regions corresponding to the spectrum of the surface of the mineral oil (0 µm in the z-axis) and the interface between oil and culture medium (18 µm z-axis) are shown.
Fig. 3
Fig. 3 Average Raman spectra of the following groups: culture media without embryos (KSOM and SOFaa), with embryos (SOFaaE), mineral oil and Petri dish in the 500-3200 cm−1 region.
Fig. 4
Fig. 4 Raman spectra of culture medium with embryos (SOFaaE) with their corresponding vibrational bands in the 500 to 3200 cm−1 region.
Fig. 5
Fig. 5 Principal Component Analysis (PCA) of the Raman spectra of the culture media without embryos (KSOM and SOFaa) and with embryos (SOFaaE) in the Raman bands from 500 to 1800 cm−1 (A) and from 1800 to 3200 cm−1 (B).
Fig. 6
Fig. 6 Loading plot analysis of the Raman spectra of the culture media without embryos (KSOM and SOFaa) and with embryos (SOFaaE) in the Raman bands from 500 to 1800 cm−1 (A) and from 1800 to 3200 cm−1 (B).

Tables (1)

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Table 1 Raman bands assignments of the culture medium with embryos (SOFaaE).

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