Abstract
We theoretically demonstrate a technique allowing a highly efficient observation of all single-molecule emitters in one fluorescent image, regardless of the orientations of their transition dipole moments. To perform this technique, we combine near-unity collection efficiency for an arbitrarily oriented emitter and the elimination of the excitation efficiency dependence on the emitter’s orientation. The former task is solved by placing the emitter in a special metallo-dielectric antenna [Opt. Lett. 36, 3545 (2011) [CrossRef] ]; the latter, by means of laser-scanning confocal fluorescence microscopy using elliptically polarized cylindrical vector beams [J. Exp. Theor. Phys. Lett. 97, 52 (2013) [CrossRef] ]. We present conditions under which the collection efficiency for arbitrarily oriented emitters is and the excitation efficiency of an emitter of minimally excitable orientation amounts to of the excitation efficiency of an emitter of maximally excitable orientation. These conditions are satisfied in the broad spectral range between 0.4 and 1.0 μm: the emitters can be excited with any wavelength and emit any fluorescence spectra in this range. The presented results are based on simulation data.
© 2015 Optical Society of America
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