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Mapping GFP Structural Evolution during Excited-State Proton Transfer with Femtosecond Stimulated Raman

Chong Fang, Renee R. Frontiera, Rosalie Tran, and Richard A. Mathies

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Abstract

We use femtosecond stimulated Raman spectroscopy to view transient structural changes of the photoexcited GFP-chromophore, identifying low-frequency ring-wagging motions that gate excited-state proton transfer. Mechanistic insights on reactive systems require structural probing with femtosecond resolution.

© 2010 Optical Society of America

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