Abstract

In the present study we clarify the detailed mechamism of the excited state dynamics of rhodopsin followed by the formation of the primary photoproduct by femtosecond spectroscopy in 400-1000 nm probe spectral region using tunable femtosecond laser over 500-600 nm obtained by using two-stage dye amplifiers of wavelength-selected femtosecond continuum. The experiment has improved the data dramatically in time resolution in comparison with our previous work¹ and in the probe spectral region in comparison with the very recent work at U. C. Berkley². The output pulse width and energy are 300 fs, > 2µJ for spectral width of 3 nm. Octopus rhodopsin solubilized by detergent(L-1690 or SM-1200) in H₂O or D₂O was used as samples.

© 1992 The Author(s)