Calibrated fMRI permits estimation of relative changes in the Cerebral Metabolic Rate of Oxygen Consumption (rCMRO2) from combined BOLD and ASL measurements during a functional task. Here, we improved the accuracy of the approach by using Two-Photon microscopic measurements of the cortical microvasculature together with first principle Monte Carlo simulations of proton diffusion across the two-photon volumes. Our method permitted us (1) to validate Calibrated fMRI from the microscopic point of view and (2) to optimize the free parameters of the biophysical model assumed, therefore increasing the accuracy of this method to estimate rCMRO2.

© 2016 Optical Society of America

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