3D Histology of Tissue using Vibrating Microtome Block- Face Imaging and MUSE Microscopy (3D MUSE)

Farzad Fereidouni; Laura Wood; Ashley Kiemen; Tanishq Abraham; Pei-Hsun Wu; Denis Wirtz; Denis Wirtz; Denis Wirtz; Richard Levenson

doi:10.1364/NTM.2021.NW3C.4

Biophotonics Congress 2021
OSA Technical Digest (Optica Publishing Group, 2021),
paper NW3C.4
•https://doi.org/10.1364/NTM.2021.NW3C.4

3D Histology of Tissue using Vibrating Microtome Block- Face Imaging and MUSE Microscopy (3D MUSE)

Farzad Fereidouni, Laura Wood, Ashley Kiemen, Tanishq Abraham, Pei-Hsun Wu, Denis Wirtz, and Richard Levenson

Novel Techniques in Microscopy 2021

Washington, DC United States
12–16 April 2021
ISBN: 978-1-943580-85-9

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Histological and clinical techniques (NW3C)

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F. Fereidouni, L. Wood, A. Kiemen, T. Abraham, P. Wu, D. Wirtz, and R. Levenson, "3D Histology of Tissue using Vibrating Microtome Block- Face Imaging and MUSE Microscopy (3D MUSE)," in Biophotonics Congress 2021, C. Boudoux, K. Maitland, C. Hendon, M. Wojtkowski, K. Quinn, M. Schanne-Klein, N. Durr, D. Elson, F. Cichos, L. Oddershede, V. Emiliani, O. Maragò, S. Nic Chormaic, N. Pégard, S. Gibbs, S. Vinogradov, M. Niedre, K. Samkoe, A. Devor, D. Peterka, P. Blinder, and E. Buckley, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper NW3C.4.

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Abstract

We describe 3-dimensional histology of fresh or fixed unimbedded tissue using a vibrating microtome with MUSE (microscopy with UV surface excitation) to acquire block-face images that can be reconstructed into 3-dimensional data sets.

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