3D Large-volume Histological Imaging of Ex vivo Tissue Samples Using Inverted Selective Plane Illumination Microscopy (iSPIM)

Bihe Hu; Guang Li; J. Quincy Brown

doi:10.1117/12.2526875

Clinical and Preclinical Optical Diagnostics II
SPIE Proceedings (Optica Publishing Group, 2019),
paper 11073_19
•https://doi.org/10.1117/12.2526875

3D Large-volume Histological Imaging of Ex vivo Tissue Samples Using Inverted Selective Plane Illumination Microscopy (iSPIM)

Bihe Hu, Guang Li, and J. Quincy Brown

European Conference on Biomedical Optics 2019

Munich Germany
23–25 June 2019
ISBN: 9781510628397

From the session
Novel Methods for Cell and Tissue Analysis (NMCTA)

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B. Hu, G. Li, and J. Q. Brown, "3D Large-volume Histological Imaging of Ex vivo Tissue Samples Using Inverted Selective Plane Illumination Microscopy (iSPIM)," in Clinical and Preclinical Optical Diagnostics II, Vol. EB101 of SPIE Proceedings (Optica Publishing Group, 2019), paper 11073_19.

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Abstract

The use of iSPIM and optical-clearing enables 3D high-content histological imaging of large tissues with subcellular resolution and almost 2 mm imaging depth, providing 3D structural information for generating tissue models or for validating low-resolution imaging modalities.

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