Abstract

We present an optical system combining digital holography or generalized phase contrast with temporal focusing for the generation of two-photon excitation patterns with arbitrary shapes and micrometer axial resolution. We show that temporal focusing is remarkably resilient to scattering, maintaining desired spatial excitation profiles irrespective of the details of sample scattering. Combining temporal focusing with generalized phase contrast, we demonstrated efficient in depth-photoactivation of optogenetics proteins in brain slices.

© 2013 Optical Society of America