Abstract

The powerful neuroscientific discipline of optogenetics can be described as the branch of biotechnology which combines genetic engineering with optics to observe and control the function of genetically targeted groups of cells with light. Using advanced volumetric laser beam-shaping such as new Deep Learning-based Computer Generated Holography (CGH) makes it possible to take advantage of cutting-edge two-photon technology to develop an unprecedented ‘circuit optogenetics’ platform with both high spatio-temporal selectivity and high penetration depth without disturbing speckle-noise in living neural tissue. We will discuss our most recent CGH-encoding modalities designed for fully parallel circuit optogenetics.

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