Abstract

We are applying optical matching techniques to two types of applications: (1) the accurate determination of tissue optical properties and (2) improving in vivo imaging quality. With optical matching, measurement of tissue optical properties to accuracies of a few percent is possible. Accurate tissue properties are useful for physiological monitoring, modeling optical imaging and therapies, and providing a priori information for image reconstruction. For imaging, matching improves illumination uniformity and contrast, and makes better use of image dynamic range.

In a separate effort, we are developing a new class of reporters which provide upconversion from molecular labels. Advantages of these materials include no autofluorescence, multiplexing capabilities, no photobleaching, and excitation with compact and low power diode lasers. Applications include in vitro assays and in vivo imaging.

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