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Conferences > FiO > 2015 > FTu2D > FTu2D.5

Hyperspectral Multiphoton Microscopy: Demonstration of 48-channel Imaging In Vivo

A. J. Bares, S. Tilley, P. C. Doerschuk, and C. B. Schaffer

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Abstract

We constructed a hyperspectral multiphoton microscope that records 48 channels of excitation/emission spectral data per pixel, while remaining insensitive to optical scattering. We demonstrate imaging of multiple spectrally overlapping fluorophores in mouse cortex, in vivo.

© 2015 Optical Society of America

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