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Conferences > ECBO > 2023 > ITCA > 126320G
  • Optical Coherence Imaging Techniques and Imaging in Scattering Media V
  • Technical Digest Series (Optica Publishing Group, 2023),
  • paper 126320G
  • •https://doi.org/10.1117/12.2675002

Morphologic analysis of the human tympanic membrane by polarization-sensitive OCT and polarized light microscopy

Svea Steuer, Joseph Morgenstern, Anett Jannasch, Lars Kirsten, Edmund Koch, Marcus Neudert, and Jonas Golde

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Abstract

The collagen layer of the human tympanic membrane (TM), which is essential for sound conduction and hearing, can be assessed in vivo by using endoscopic polarization-sensitive optical coherence tomography (PS-OCT), as we have shown recently. Since the resolution of the utilized system was limited, an ex vivo validation of the structural information is presented here. By comparing higher resolution PS-OCT images of a temporal bone specimen and polarized light microscopy (PLM) of histological slices from that TM with picrosirius red staining, the microstructure is investigated.

© 2023 SPIE

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