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A novel adaptive optics illumination device for in vivo imaging of fluorescently labeled specimens

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Abstract

We developed a small form factor, microscope objective like, wavefront shaping assisted optical device (Wavelens) to produce and control illumination patterns for use on Light-Sheet Fluorescence Microcopy (LSFM). We characterize it and demonstrate its capability to perform in vivo imaging using fluorescently labeled specimens, outperforming conventional optics.

© 2023 SPIE

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