Abstract

Brillouin microscopy has emerged as a non-invasive and label-free technique to map micro-mechanical properties of cells. Here we apply Brillouin microscopy to probe reorganization of F-actin network in respiratory cells treated with Timothy grass pollen protein extracts. The results of our measurements in conjunction with clustering data analysis confirm spatial cellular reorganization of F-actin proteins and compromised junctional integrity in treated cells as compared to controls.

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