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Conferences > NTM > 2017 > NTu2C > NTu1C.3

Optimization of Optical Sectioning Performance in Thick Tissue Imaging with Stage-Scanning Inverted Selective Plane Illumination Microscopy (iSPIM)

Bihe Hu and J. Quincy Brown

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Abstract

Imaging performance of iSPIM in fluorescently-stained intact human tissues has been optimized by combining with structured illumination or electronic confocal slit detection, or both. SI achieved the highest SBR in propidium iodide-stained prostate.

© 2017 Optical Society of America

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