Adaptive Holographic Optogenetic Illumination for Human Neural Network Analysis

F. Schmieder; R. Habibey; V. Busskamp; J.W. Czarske; J.W. Czarske; J.W. Czarske; L. Büttner; L. Büttner

doi:10.1364/DH.2022.W4A.7

Digital Holography and 3-D Imaging 2022
Technical Digest Series (Optica Publishing Group, 2022),
paper W4A.7
•https://doi.org/10.1364/DH.2022.W4A.7

Adaptive Holographic Optogenetic Illumination for Human Neural Network Analysis

F. Schmieder, R. Habibey, V. Busskamp, J.W. Czarske, and L. Büttner

Digital Holography and Three-Dimensional Imaging 2022

Cambridge United Kingdom
1–4 August 2022
ISBN: 978-1-957171-12-8

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Digital Holographic Techniques for Bio-imaging II (W4A)

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F. Schmieder, R. Habibey, V. Busskamp, J. W. Czarske, and L. Büttner, "Adaptive Holographic Optogenetic Illumination for Human Neural Network Analysis," in Digital Holography and 3-D Imaging 2022, D. Chu, J. Park, C. Cheng, and P. Ferraro, eds., Technical Digest Series (Optica Publishing Group, 2022), paper W4A.7.

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Abstract

A two-wavelength optogenetic stimulation platform with high spatiotemporal resolution and inherent aberration correction is presented. By stimulating single neurons, we investigated the temporal evolution of connectivity in human induced pluripotent stem cell-derived neuronal networks in-vitro.

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