Abstract

Here we present the patented Synchronous Red Green Blue – Fluorescence lifetime imaging microscope (SyncRGB-FLIM) method [1,2], an extension of the conventional Multiphoton Fluorescence Lifetime Imaging Microscopy (MP-FLIM) method, with the important difference of deploying a sub-10 fs ultra-broadband (>400 nm bandwidth) laser capable of simultaneous efficient excitation of spectrally well-separated dyes or autofluorescence molecules. Equipped with fixed beam stage scanning, this method was used to image both fixed multi-labeled samples and to retrieve metabolic imaging of both 2D live cell models [3,4].

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