Few-cycle ultra-broadband beam scanning microscope prototype

Christian Maibohm; Hugo Sebastião; João Martins; Maria Leonor Ribeiro; Miguel Miranda; Paulo Tiago Guerreiro; Rosa Romero; Helder Crespo; Helder Crespo; Jana Berit Nieder

Conference on Lasers and Electro-Optics/Europe (CLEO/Europe 2023) and European Quantum Electronics Conference (EQEC 2023)
Technical Digest Series (Optica Publishing Group, 2023),
paper cd_p_18

Few-cycle ultra-broadband beam scanning microscope prototype

Christian Maibohm, Hugo Sebastião, João Martins, Maria Leonor Ribeiro, Miguel Miranda, Paulo Tiago Guerreiro, Rosa Romero, Helder Crespo, and Jana Berit Nieder

The European Conference on Lasers and Electro-Optics 2023

Munich Germany
26–30 June 2023
ISBN: 979-8-3503-4599-5

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C. Maibohm, H. Sebastião, J. Martins, M. L. Ribeiro, M. Miranda, P. T. Guerreiro, R. Romero, H. Crespo, and J. B. Nieder, "Few-cycle ultra-broadband beam scanning microscope prototype," in Conference on Lasers and Electro-Optics/Europe (CLEO/Europe 2023) and European Quantum Electronics Conference (EQEC 2023), Technical Digest Series (Optica Publishing Group, 2023), paper cd_p_18.

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Abstract

Multiphoton microscopy (MPM) has long been a corner stone in the field of bioimaging spearheading deep tissue, and label-free imaging. The inherit sectioning capability, and reduced photo-induced damage makes MPM an ideal tool for the study of thick live samples related to clinical studies. The ExtreMed (Extreme Ultrashort Pulses for Advanced Medical Applications and Diagnostics) project [*], is on the forefront in the exploration of MPM with the utilization of a few-cycle ultra-broadband sub-10 fs pulsed laser and applying the patented SyncRGB-FLIM (synchronous Red-Green-Blue fluorescence lifetime imaging microscopy) method [1, 2].

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