Differentiable Adaptive Optics and Light Shaping in Two-Photon Microscopy

Ivan Vishniakou; Johannes D. Seelig

doi:10.1364/BRAIN.2023.BW3B.5

Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)
Technical Digest Series (Optica Publishing Group, 2023),
paper BW3B.5
•https://doi.org/10.1364/BRAIN.2023.BW3B.5

Differentiable Adaptive Optics and Light Shaping in Two-Photon Microscopy

Ivan Vishniakou and Johannes D. Seelig

Optics and the Brain 2023

Vancouver, British Columbia Canada
24–27 April 2023
ISBN: 978-1-957171-21-0

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High Speed and Adaptive Microscopy (BW3B)

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I. Vishniakou and J. D. Seelig, "Differentiable Adaptive Optics and Light Shaping in Two-Photon Microscopy," in Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN), Technical Digest Series (Optica Publishing Group, 2023), paper BW3B.5.

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Abstract

We develop a differentiable optics approach for modeling light propagation through a high N.A. microscope objective based on efficient computational optimization of the Debye-Wolf diffraction integral. We apply the technique for wavefront sensing for adaptive optics without guide stars and light shaping in a two-photon microscope.

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