Differentiable Optics for Wavefront Sensing in Two-photon Fluorescence Microscopy

Ivan Vishniakou; Johannes D. Seelig

doi:10.1364/AOA.2022.OW3G.5

Imaging and Applied Optics Congress 2022 (3D, AOA, COSI, ISA, pcAOP)
Technical Digest Series (Optica Publishing Group, 2022),
paper OW3G.5
•https://doi.org/10.1364/AOA.2022.OW3G.5

Differentiable Optics for Wavefront Sensing in Two-photon Fluorescence Microscopy

Ivan Vishniakou and Johannes D. Seelig

Adaptive Optics and Applications 2022

Vancouver, British Columbia Canada
11–15 July 2022
ISBN: 978-1-957171-09-8

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Microscopy I (OW3G)

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I. Vishniakou and J. D. Seelig, "Differentiable Optics for Wavefront Sensing in Two-photon Fluorescence Microscopy," in Imaging and Applied Optics Congress 2022 (3D, AOA, COSI, ISA, pcAOP), Technical Digest Series (Optica Publishing Group, 2022), paper OW3G.5.

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Abstract

We develop a differentiable physics approach for adaptive optics in fluorescence microscopy. Image formation in the microscope is described using a vectorial Debye-Wolf model. Unknown sample aberrations are found and corrected by adjusting model parameters using gradient-based optimisation and only a small number of aberrated images.

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