Unlike traditional methods, a laser speckle is used here to illuminate the sample. A speckle is a random grainy pattern in both 2D and 3D, resulting from a laser scattered by a rough object, in this case a Scotch tape. This speckle pattern is scanned across the object to provide diversity in the recorded measurements and enable high-resolution 3D imaging across a wide field-of-view using a low-resolution objective. The resolution of this system is only limited by the speckle grain size, which can be easily made smaller using thicker Scotch tape. HT-29 cancer cells across a 314×500×24 µm3 volume were successfully imaged at 0.6×0.6×6 µm3 voxel resolution to show the potential for this technique as a low-cost solution for imaging 3D samples.
08/29/2019 7:10 AM
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