Asymmetry robust centroid localization in confocal microscopy

Cheng Chen; Cheng Chen; Cheng Chen; Changchun Chai; Xiaojun Liu; Xiangqian Jiang; Xiangqian Jiang; Wenlong Lu; Wenlong Lu

doi:10.1364/OL.455938

Optics Letters
Vol. 47,
Issue 8,
pp. 1933-1936
(2022)
•https://doi.org/10.1364/OL.455938

Asymmetry robust centroid localization in confocal microscopy

Cheng Chen, Changchun Chai, Xiaojun Liu, Xiangqian Jiang, and Wenlong Lu

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Cheng Chen, Changchun Chai, Xiaojun Liu, Xiangqian Jiang, and Wenlong Lu, "Asymmetry robust centroid localization in confocal microscopy," Opt. Lett. 47, 1933-1936 (2022)

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Table of Contents Category
- Optical Sensors, Measurements, and Metrology
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About this Article
History
- Original Manuscript: February 10, 2022
- Revised Manuscript: March 3, 2022
- Manuscript Accepted: March 3, 2022
- Published: April 5, 2022

Abstract

We present a centroid algorithm with asymmetry-robust error compensation for the peak position localization of asymmetrical axial response signals in confocal microscopy. Compared with the state-of-the-art algorithms, which are usually developed for symmetrical signals, our asymmetry robust centroid algorithm is found to have much smaller localization bias and higher precision for an asymmetrical confocal signal in numerical simulations and experiments.

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Data availability

Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

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µm	$r m s_{b i a s}$	$r m s_{s t d}$	$p v_{b i a s}$	$m a x_{s t d}$
$A R C A$	$0.0055$	$0.010$	$0.038$	$0.038$
$M L E$	$0.033$	$0.021$	$0.28$	$0.21$
$C P F A$	$0.044$	$0.034$	$0.31$	$0.13$

µm	$r m s_{b i a s}$	$r m s_{s t d}$	$p v_{b i a s}$	$m a x_{s t d}$
$A R C A$	$0.045$	$0.17$	$0.24$	$0.45$
$M L E$	$0.066$	$0.16$	$0.40$	$0.47$
$C P F A$	$0.080$	$0.18$	$0.45$	$0.42$

µm	$r m s_{b i a s}$	$r m s_{s t d}$	$p v_{b i a s}$	$m a x_{s t d}$
$A R C A$	$0.0055$	$0.010$	$0.038$	$0.038$
$M L E$	$0.033$	$0.021$	$0.28$	$0.21$
$C P F A$	$0.044$	$0.034$	$0.31$	$0.13$

µm	$r m s_{b i a s}$	$r m s_{s t d}$	$p v_{b i a s}$	$m a x_{s t d}$
$A R C A$	$0.045$	$0.17$	$0.24$	$0.45$
$M L E$	$0.066$	$0.16$	$0.40$	$0.47$
$C P F A$	$0.080$	$0.18$	$0.45$	$0.42$

Abstract

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Equations (11)

Optics Letters