Abstract

The direct imaging of tissue preserved in formalin-fixed paraffin-embedded (FFPE) blocks remains a challenge. There are presently millions of tissues preserved as FFPE blocks whose assessment via bright-field microscopes requires them to be sectioned and subsequently stained. These processes are laborious, resource-intensive, and time consuming. In this Letter, we utilize an ultraviolet laser with photoacoustic remote sensing to provide a novel method that enables direct label-free pathological assessment of FFPE blocks. We demonstrate the efficacy of this technique by imaging human breast tissue, highlighting salient features such as ducts, adipocytes, and ductal hyperplasia. This direct imaging of FFPE blocks facilitates pathological assessment much earlier in the histopathological workflow, saving valuable time in clinical and research settings. The presented non-contact label-free reflection-mode device enables augmentation of existing histopathological workflows and aims to expand the arsenal of imaging technologies available to clinicians.

© 2020 Optical Society of America

Full Article  |  PDF Article
More Like This
Label-free lipid contrast imaging using non-contact near-infrared photoacoustic remote sensing microscopy

Pradyumna Kedarisetti, Nathaniel J. M. Haven, Brendon S. Restall, Matthew T. Martell, and Roger J. Zemp
Opt. Lett. 45(16) 4559-4562 (2020)

High-speed label-free ultraviolet photoacoustic microscopy for histology-like imaging of unprocessed biological tissues

Xiufeng Li, Lei Kang, Yan Zhang, and Terence T. W. Wong
Opt. Lett. 45(19) 5401-5404 (2020)

Label-free, non-contact, in vivo ophthalmic imaging using photoacoustic remote sensing microscopy

Zohreh Hosseinaee, Layla Khalili, James A. Tummon Simmons, Kevan Bell, and Parsin Haji Reza
Opt. Lett. 45(22) 6254-6257 (2020)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription