Abstract

We demonstrate stain-free, high-contrast, subcellular-resolution imaging of astroglial cells using epi-detected third-harmonic generation (THG). The astrocyte-imaging capability of THG is verified by colocalizing THG images with fluorescence images of astrocytes expressing a genetically encodable fluorescent reporter. We show that THG imaging with an optimized point-spread function can reliably detect significant subcellular features of astrocytes, including cell nuclei, as well as the soma shape and boundaries.

© 2019 Optical Society of America

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Supplementary Material (1)

NameDescription
» Visualization 1       Stacks of TPEF and THG images taken by scanning the focus of the laser beam along the z-axis through layers V–VI in rat’s cortex.

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