Abstract

We introduce a fiber-based laser system providing 130 fs pulses with 3.5 nJ energy at 920 nm at a 43 MHz repetition rate and illustrate the potential of the source for two-photon excited fluorescence microscopy of living mouse brain. The laser source is based on frequency-doubling high-energy solitons generated and frequency-shifted to 1840 nm in large mode area fibers. This simple laser system could unleash the potential of two-photon microscopy techniques in the biology laboratory where green fluorescent proteins with two-photon absorption spectrum peaking around 920 nm are routinely used.

© 2018 Optical Society of America

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Supplementary Material (3)

NameDescription
» Visualization 1       Animated 240 μm thick volume stack (60 slices, 4 μm z-step) showing deep imaging on an eGFP mouse brain.
» Visualization 2       Animated 200 μm thick z-stack (51 slices, 4 μm z-step) showing deep imaging in the subcutaneous tissue of an e-GFP mouse head.
» Visualization 3       Animated 200 μm thick z-stack (51 slices, 4 μm z-step) showing imaging through the right parietal bone of an e-GFP mouse head.

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