Abstract

Acetylcholine is a prominent neurotransmitter involved in regulation of behavioral activities in human beings. The dysfunction in acetylcholine regulation is linked with several neurological disorders including Alzheimer's disease. This study presents the fabrication and characterization of an optical biosensor for detection of acetylcholine utilizing fiber-optic surface plasmon resonance sensing technology. The sensing probe comprises of multilayers of silver metal and tantalum (v) oxide (Ta2O5) nanoflakes functionalized with acetylcholinesterase enzyme over unclad core of an optical fiber. An exposure of the sensing probe to acetylcholine solutions culminates in the modification of refractive index of the sensing surface. Operating in wavelength interrogation mode, a blue shift of 44 nm is achieved in resonance wavelength for the change in acetylcholine concentration from 0 to 10 μM. Under optimized conditions of experimental parameters, the biosensor in concern, revealed an appreciable sensitivity of 8.709 nm/μM and a remarkable LOD value of 38 nM in addition to being highly selective toward acetylcholine. The biosensor is endowed with various advantages, such as ease of handling, compactness, and cost efficacy along with the capability of online monitoring and remote sensing.

© 2018 IEEE

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