Abstract

This manuscript is a continuation of a recent study that described the use of fully integrated gas chromatography with direct deposition Fourier transform infrared detection and mass spectrometric detection (GC-FT-IR-MS) to identify and confirm the presence of sibutramine and AB-FUBINACA. The purpose of the current study was to employ the GC-FT-IR portion of the same instrument to quantify these compounds, thereby demonstrating the ability to identify, confirm, and quantify drug substances using a single GC-FT-IR-MS unit. The performance of the instrument was evaluated by comparing quantitative analytical figures of merit to those measured using an established, widely employed method for quantifying drug substances, high performance liquid chromatography with ultraviolet detection (HPLC-UV). The results demonstrated that GC-FT-IR was outperformed by HPLC-UV with regard to sensitivity, precision, and linear dynamic range (LDR). However, sibutramine and AB-FUBINACA concentrations measured using GC-FT-IR were not significantly different at the 95% confidence interval compared to those measured using HPLC-UV, which demonstrates promise for using GC-FT-IR as a semi-quantitative tool at the very least. The most significant advantage of GC-FT-IR compared to HPLC-UV is selectivity; a higher level of confidence regarding the identity of the analyte being quantified is achieved using GC-FT-IR. Additional advantages of using a single GC-FT-IR-MS instrument for identification, confirmation, and quantification are efficiency, increased sample throughput, decreased consumption of laboratory resources (solvents, chemicals, consumables, etc.), and thus cost.

© 2018 The Author(s)

Detection, identification, and estimation of biological aerosols and vapors with a Fourier-transform infrared spectrometer

Avishai Ben-David and Hsuan Ren
Appl. Opt. 42(24) 4887-4900 (2003)

Nonlinear interferometer for Fourier-transform mid-infrared gas spectroscopy using near-infrared detection

Chiara Lindner, Jachin Kunz, Simon J. Herr, Sebastian Wolf, Jens Kießling, and Frank Kühnemann
Opt. Express 29(3) 4035-4047 (2021)

Ultraviolet laser microplasma–gas chromatography detector: detection of species-specific fragment emission

Randy J. Locke, Jeffrey B. Morris, Brad E. Forch, and Andrzej W. Miziolek
Appl. Opt. 29(33) 4987-4992 (1990)

Detection and identification of drugs under real conditions by using noisy terahertz broadband pulse

Vyacheslav A. Trofimov and Svetlana A. Varentsova
Appl. Opt. 55(33) 9605-9618 (2016)

Accurate infrared transmittance measurements on optical filters using an FT-IR spectrometer

David A. C. Compton, John Drab, and Howard S. Barr
Appl. Opt. 29(19) 2908-2912 (1990)