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  • Applied Spectroscopy
  • Vol. 56,
  • Issue 3,
  • pp. 312-317
  • (2002)

Direct Identification and Quantitative Determination of Acidic and Neutral Diterpenes Using 13C-NMR Spectroscopy. Application to the Analysis of Oleoresin of Pinus nigra

Serge Rezzi, Ange Bighelli, Vincent Castola, and Joseph Casanova

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Abstract

We report a methodology that allows identification and quantitative determination of individual acidic and neutral diterpenes in natural mixtures, using the computer-aided analysis of their <sup>13</sup>C-NMR spectra. The analytical procedure was validated on artificial mixtures and then applied to authentic oleoresins of <i>Pinus nigra</i>. Up to eleven diterpenes, which represented 63% of the total composition of the oleoresin, were identified and quantified without previous separation or derivatization.

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