Abstract

Efficient detector optics incorporating a hyperhemispherical element have been developed to facilitate the coupling of capillary electrophoresis or microchromatography with Raman spectroscopy. The design has a high tolerance for alignment and focusing errors. A simple model has been developed to predict the behavior of the system as capillary diameter is varied. This approach was found to provide good qualitative agreement with experiment. Limits of detection in capillary electrophoresis experiments without preconcentration were found to be ~ 5 mM for nitrate and perchlorate ions. Direct detection of several carbohydrates was also demonstrated; with the use of sample stacking, limits of detection were in the millimolar range. The analytes were distinguishable by their Raman spectra.

Laser-induced fluorescence detector with a fiber-coupled micro GRIN lens for capillary electrophoresis

Oluwatosin O. Dada
Appl. Opt. 59(16) 4849-4855 (2020)

On-column refractive-index detection based on retroreflected beam interference for capillary electrophoresis

Yanzhuo Deng and Bincheng Li
Appl. Opt. 37(6) 998-1005 (1998)

Terahertz-capillary electrophoresis (THz-CE) for direct detection of separated substances in solutions

Keiko Kitagishi, Takayuki Kawai, Masayoshi Tonouchi, and Kazunori Serita
Opt. Mater. Express 14(2) 472-482 (2024)

Fluorescence monitoring of microchip capillary electrophoresis separation with monolithically integrated waveguides

Chaitanya Dongre, Ronald Dekker, Hugo J. W. M. Hoekstra, Markus Pollnau, Rebeca Martinez-Vazquez, Roberto Osellame, Giulio Cerullo, Roberta Ramponi, Rob van Weeghel, Geert A. J. Besselink, and Hans H. van den Vlekkert
Opt. Lett. 33(21) 2503-2505 (2008)

Laser-based refractive-index detection for capillary electrophoresis: ray-tracing interference theory

Beat Krattiger, Alfredo E. Bruno, H. Michael Widmer, Martial Geiser, and René Dändliker
Appl. Opt. 32(6) 956-965 (1993)