Abstract

Total internal reflection fluorescence (TIRF) is developed as a surface selective method to allow the environment of a liquid/solid interface to be probed by fluorescent molecules which are adsorbed from solution. The method has been used to detect pyrene sorbed to an octadecylsilane-derivatized fused-silica plate and resolve its spectral emission so that vibronic intensity ratios can be calculated and the surface environment characterized. Adsorption equilibria of the fluorescent probe to the surface and the depth of penetration of the evanescent excitation beam provide the basis for predicting interference from probe molecules in the solution phase. These predictions were validated by replacing the solution overlaying the alkylated silica interface with saturated vapor and comparing the apparent surface environments.

Direct studies of liquid flows near solid surfaces by total internal reflection fluorescence cross-correlation spectroscopy

Stoyan Yordanov, Andreas Best, Hans-Jürgen Butt, and Kaloian Koynov
Opt. Express 17(23) 21149-21158 (2009)

Total internal reflection fluorescence correlation spectroscopy (TIR-FCS) with low background and high count-rate per molecule

Kai Hassler, Marcel Leutenegger, Per Rigler, Ramachandra Rao, Rudolf Rigler, Michael Gösch, and Theo Lasser
Opt. Express 13(19) 7415-7423 (2005)

Absorption effects on total-internal-reflection fluorescence spectroscopy

Minoru Toriumi, Masatoshi Yanagimachi, and Hiroshi Masuhara
Appl. Opt. 31(30) 6376-6382 (1992)

Around-the-objective total internal reflection fluorescence microscopy

Thomas P. Burghardt, Andrew D. Hipp, and Katalin Ajtai
Appl. Opt. 48(32) 6120-6131 (2009)

Direct characterization of the evanescent field in objective-type total internal reflection fluorescence microscopy

Christian Niederauer, Philipp Blumhardt, Jonas Mücksch, Michael Heymann, Armin Lambacher, and Petra Schwille
Opt. Express 26(16) 20492-20506 (2018)