Abstract
Theoretical aspects of the implementation of fluorescence lifetime selectivity in multifrequency phase-resolved fluorescence spectroscopy (PRFS) are discussed. The dependence of phase-resolved fluorescence intensities on the dynamic instrumental parameters of excitation modulation frequency and detector phase angle, as well as on fluorescence lifetime, is described. The different types of fluorescence lifetime "filtering" that can be effectively achieved include longpass, shortpass, and bandpass functions. The ability to continuously scan both modulation frequency and detector phase angle greatly increases the potential of PRFS for fluorimetric analysis.
PDF Article
More Like This
Cited By
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access Optica Member Subscription