Abstract

Compared with conventional light microscopy (LM), confocal laser scan microscopy (CLSM) is characterized in part by increased light sensitivity and higher spatial resolution. These characteristics permit the detection and discrimination of minor changes in cells and tissues in a more specific, sensitive, and accurate way. Potential clinical applications related to these characteristics of CLSM are discussed. Preliminary data indicate that the higher light sensitivity of CLSM allows detection of low amounts of proto-oncogene mRNA which are minimally detectable by LM. Evaluation of Grimelius-stained sections of lung cancer by CLSM with antiflex permits detection of previously undetected granula. The higher spatial resolution of CLSM may prove to be essential for accurate assessment of nuclear shape, which is of prognostic importance in tumors of a variety of organs. When applied to cancer research and diagnosis or other relevant fields, CLSM allows improved insight in tumor cell biology and may lead to improved diagnostic pathology. Consequently, the use of CLSM could lead to more refined therapeutic indications.

© 1987 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Laser scanning fluorescence microscopy

Johan S. Ploem
Appl. Opt. 26(16) 3226-3231 (1987)

Image DNA-index (ploidy) analysis in cancer diagnosis

P. S. Oud, A. G. J. M. Hanselaar, M. M. M. Pahlplatz, J. W. R. Meijer, and G. Peter Vooijs
Appl. Opt. 26(16) 3349-3355 (1987)

Video-rate confocal scanning laser microscope for imaging human tissues in vivo

Milind Rajadhyaksha, R. Rox Anderson, and Robert H. Webb
Appl. Opt. 38(10) 2105-2115 (1999)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription