Abstract
In the confocal mode, Raman microspectroscopy can profile the biochemical content of biological cells at a subcellular level, and any changes to it by exogenous agents, such as therapeutic drugs or toxicants. As an exploration of the potential of the technique as a high-content, label-free analysis technique, this report reviews work to monitor the spectroscopic signatures associated with the uptake and response pathways of commercial chemotherapeutic agents and polymeric nanoparticles by human lung cells. It is demonstrated that the signatures are reproducible and characteristic of the cellular event, and can be used, for example, to identify the mode of action of the agent as well as the subsequent cell death pathway, and even mechanisms of cellular resistance. Data mining approaches are discussed and a spectralomics approach is proposed.
© 2018 Optical Society of America
Full Article | PDF ArticleMore Like This
Tobias J. Moritz, Douglas S. Taylor, Denise M. Krol, John Fritch, and James W. Chan
Biomed. Opt. Express 1(4) 1138-1147 (2010)
Sufang Qiu, Miaomiao Li, Jun Liu, Xiaochuan Chen, Ting Lin, Yunchao Xu, Yang Chen, Youliang Weng, Yuhui Pan, Shangyuan Feng, Xiandong Lin, Lurong Zhang, and Duo Lin
Biomed. Opt. Express 11(4) 1819-1833 (2020)
Nebras Alattar, Hasbullah Daud, Rasoul Al-Majmaie, Domonic Zeulla, Mohameed Al-Rubeai, and James H. Rice
Appl. Opt. 57(22) E184-E189 (2018)