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Conferences > UFO > 2023 > P2 > P2.26

Ultrafast 1.8 µm fiber laser system for in vivo three- and four-photon fluorescence microscopy

Takao Fuji, Hiromi H. Ueda, Ryuichiro Goto, Kosuke Hamada, Yutaro Nagasawa, and Hideji Murakoshi

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Abstract

We have developed a fiber laser system that produces 142 fs pulses at 1.8 µm and demonstrated in vivo three/four-photon fluorescence microscopy. We observe cortical neurons at a depth of 0.8 mm from the brain surface by three-photon excitation and astrocytes at a depth of 0.2 mm by four-photon excitation.

© 2023 UFOXIII

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