Abstract

An integrated microscopy system has been developed that is capable of acquiring both reflectance confocal microscope (RCM) imaging and two photon fluorescence (TPF) imaging real-time as well as Raman spectroscopy measurement of a single cell. The TPM fluorescence image of FM1-43 labeled cell membrane and the corresponding Hoechst-labelled nucleotide distribution in the Hut78 cell were obtained in combination with Raman spectroscopy measurement. A CW diode laser at 785nm was used for both trapping and Raman excitation of a living suspended cell. A femtosecond (fs) Ti: Sappire laser was used for multiphoton microscope imaging. The integrated microscopy system may provide a useful tool for understanding the fundamental cell processes and for diagnosis of cell carcinogenesis.

© 2015 Optical Society of America