Multilayer and Three-dimensional Super-Resolution Imaging of Thick Biological Samples

Alipasha Vaziri; Jianyong Tang; Hari Shroff; Charles V. Shank

doi:10.1364/NTM.2009.NMB1

Advances in Imaging
OSA Technical Digest (CD) (Optica Publishing Group, 2009),
paper NMB1
•https://doi.org/10.1364/NTM.2009.NMB1

Multilayer and Three-dimensional Super-Resolution Imaging of Thick Biological Samples

Alipasha Vaziri, Jianyong Tang, Hari Shroff, and Charles V. Shank

Novel Techniques in Microscopy 2009

Vancouver Canada
26–30 April 2009
ISBN: 978-1-55752-871-1

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A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, "Multilayer and Three-dimensional Super-Resolution Imaging of Thick Biological Samples," in Advances in Imaging, OSA Technical Digest (CD) (Optica Publishing Group, 2009), paper NMB1.

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Abstract

We have demonstrated super-resolution imaging of protein distributions in cells at depth at multiple layers with a lateral localization precision better than 50nm. The approach is based on combining photoactivated localization microscopy with temporal focusing.

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